Sugiyama H, Moberg L J, Messer S L
Appl Environ Microbiol. 1977 Apr;33(4):963-6. doi: 10.1128/aem.33.4.963-966.1977.
A modified protocol for the preparation of botulinum type A toxic crystals is decribed. The essential change was the substitution of chromatography on diethylaminoethyl-Sephadex for the step in which toxin was precipitated with ethanol. Colored materials and nucleic acids were removed by the chromatography so that crystal lots of greater uniformity could be produced. Significantly more of toxin in the starting culture was recovered as crystals. The lots of crystals obtained initially by either the original or the modified method contained an antigen that was eliminated when the lots were recrystallized.
描述了一种制备A型肉毒杆菌毒素晶体的改良方案。主要变化是用二乙氨基乙基-葡聚糖凝胶柱色谱法替代了用乙醇沉淀毒素的步骤。通过色谱法去除了有色物质和核酸，从而能够生产出更均匀的晶体批次。起始培养物中回收得到的晶体形式的毒素显著增多。最初用原始方法或改良方法获得的晶体批次都含有一种抗原，当这些批次进行重结晶时该抗原被去除。
