不同发酵条件下A型肉毒梭菌的毒素产生情况。
Toxin production by Clostridium botulinum type A under various fermentation conditions.
作者信息
Siegel L S, Metzger J F
出版信息
Appl Environ Microbiol. 1979 Oct;38(4):606-11. doi: 10.1128/aem.38.4.606-611.1979.
Abstract
The time of appearance and the quantity of toxin produced by the Hall strain of Clostridium botulinum type A were examined under various conditions. A 70-liter fermentor and a complex medium consisting of 2% casein hydrolysate and 1% yeast extract plus an appropriate concentration of glucose were employed. Optimal conditions for toxin production were as follows: a nitrogen overlay at a rate of 5 liters/min, an agitation rate of 50 rpm, a temperature of 35 degrees C, and an initial glucose concentration of 1.0% with the pH uncontrolled. Under these conditions, the maximum toxin concentration (6.3 x 10(5) mouse median lethal doses/ml) was attained within 24 h. Cell lysis was apparently not required to obtain maximum toxin concentrations under the fermentation conditions described.
摘要
在不同条件下检测了A型肉毒梭菌霍尔菌株产生毒素的时间和产量。使用了一个70升的发酵罐和一种由2%酪蛋白水解物、1%酵母提取物以及适当浓度葡萄糖组成的复合培养基。毒素产生的最佳条件如下：氮气覆盖速率为5升/分钟，搅拌速率为50转/分钟，温度为35℃，初始葡萄糖浓度为1.0%，pH值不控制。在这些条件下，24小时内可达到最大毒素浓度（6.3×10⁵小鼠半数致死剂量/毫升）。在所描述的发酵条件下，显然不需要细胞裂解就能获得最大毒素浓度。
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