Ding Dingbang, Wang Hua, Liang Jiajun, Su Chao, Zhang Minjuan, Jiao Feng, Bao Lijun, Wang Xia
College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Henan Sericulture Research Institute, Zhengzhou, 450003, Henan, China.
Sci Rep. 2025 Jul 2;15(1):23031. doi: 10.1038/s41598-025-05528-6.
Inonotus hispidus, a fungal species morphologically closely related to the medicinal genus Sanghuang,has garned considerable attention due to its potential health-prompting benefits. This study presents the whole-genome sequence of I. hispidus ZA-14, a monokaryotic strain isolated from wild fruiting bodies growing in Morus alba (hereinafter referred to as MA). The 37.68 Mb genome was assembled from 5771 Mb of raw data, resulting in 51 contigs with a GC content of 48.48%. A total of 8924 protein-coding genes were predicted, with 223 non-coding RNAs identified. The genome analysis revealed a high density of genes associated with secondary metabolite biosynthesis, including those involved in terpenoid, polysaccharide, and flavonoid pathways-key contributors to its medicinal attributes. Comparative genomic analysis with other related fungal species provided insights into the evolutionary dynamics and speciation of I. hispidus. The study also identified a significant enrichment of glycoside hydrolase family genes, suggesting a critical role in nutrient absorption and utilization. These findings enhance our understanding of the genetic basis of I. hispidus's medicinal value and its evolutionary relationships, providing a foundation for future breeding.
糙皮纤孔菌是一种在形态上与药用真菌桑黄属密切相关的真菌物种，因其潜在的促进健康的益处而备受关注。本研究展示了糙皮纤孔菌ZA - 14的全基因组序列，ZA - 14是从生长在桑树上的野生子实体中分离出的单核菌株（以下简称MA）。该基因组大小为37.68 Mb，由5771 Mb的原始数据组装而成，共产生51个重叠群，GC含量为48.48%。共预测出8924个蛋白质编码基因，鉴定出223个非编码RNA。基因组分析显示与次生代谢物生物合成相关的基因密度很高，包括参与萜类、多糖和黄酮类途径的基因——这些是其药用特性的关键贡献者。与其他相关真菌物种的比较基因组分析为糙皮纤孔菌的进化动态和物种形成提供了见解。该研究还发现糖苷水解酶家族基因显著富集，表明其在营养吸收和利用中起关键作用。这些发现加深了我们对糙皮纤孔菌药用价值的遗传基础及其进化关系的理解，为未来的育种提供了基础。
