B Sunantha, Nagarajan Praveen, Sudhakumar J, Thomas Blessen Skariah, Sankaranarayanan Pooja, Pitchaikani Sasikumar, T V Suchithra
Department of Civil Engineering, National Institute of Technology Calicut, Kozhikode, Kerala, India.
Department of Bio Science and Engineering, National Institute of Technology Calicut, Kozhikode, Kerala, India.
Sci Rep. 2025 Jul 1;15(1):20917. doi: 10.1038/s41598-025-94641-7.
Self-healing mechanisms based on calcite precipitation have proven effective in enhancing concrete's strength, durability, and crack repair capabilities. This research explores the self-healing process in concrete via microbial-induced calcium carbonate precipitation (MICP) using Bacillus cereus and Vibrio natriegens. This study involved various experimental analysis such as biochemical and morphological identification, bacterial growth evaluation, and characterization of mortar specimens using energy-dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), testing for ultrasonic pulse velocity (UPV) and compressive strength testing. The findings revealed that the bacterial consortium outperformed the individual bacterial strains, resulting in a 19.85% increase in compressive strength and a 29.6% decrease in water absorption compared to conventional mortar. XRD and EDS analyses confirmed the formation of calcium carbonates within the cracks. These results position the bacterial consortium as an effective and economically viable approach for bio-self-healing concrete through MICP, especially in hot and humid environments.
基于方解石沉淀的自修复机制已被证明能有效提高混凝土的强度、耐久性和裂缝修复能力。本研究通过使用蜡状芽孢杆菌和纳氏弧菌的微生物诱导碳酸钙沉淀（MICP）来探索混凝土中的自修复过程。该研究涉及各种实验分析，如生化和形态鉴定、细菌生长评估，以及使用能量色散X射线光谱（EDS）、X射线衍射（XRD）对砂浆试件进行表征、超声脉冲速度（UPV）测试和抗压强度测试。研究结果表明，细菌联合体的性能优于单个细菌菌株，与传统砂浆相比，抗压强度提高了19.85%，吸水率降低了29.6%。XRD和EDS分析证实了裂缝内碳酸钙的形成。这些结果表明，细菌联合体是一种通过MICP实现生物自修复混凝土的有效且经济可行的方法，尤其是在炎热潮湿的环境中。
