el-Bayar H, Steel L, Montcalm E, Danquechin-Dorval E, Dubois A, Shea-Donohue T
Prostaglandins. 1985 Sep;30(3):401-17. doi: 10.1016/0090-6980(85)90115-7.
Prostaglandins (PG) are known to alter a variety of gastrointestinal functions, but the physiological role of endogenous PG remains unclear. This experiment was designed to evaluate changes in gastric secretion following both acute and chronic inhibition of PG synthesis with indomethacin (5 mg/kg s.c.). Gastric juice was collected by continuous aspiration in 8 conscious chair-adapted male rhesus monkeys following treatment with saline or indomethacin for one or four days. The gastric juice was analyzed for H+, Na+, K+, and Cl- concentrations. The amount of soluble mucus in the gastric juice was estimated using Alcian Blue dye binding of acidic glycoproteins and Periodic Acid Schiff reaction with neutral glycoproteins. PG levels were measured in the plasma and in biopsy samples of fundus, antrum and duodenum. Both one and four days of indomethacin significantly (p less than 0.05) decreased tissue PG levels in the fundus, antrum and duodenum. Plasma levels of PGF2 alpha were significantly (p less than 0.05) decreased after both one and four days of indomethacin, while PGE2 and 6-keto PGF1 alpha were significantly inhibited only after four days of indomethacin. Both acute and chronic inhibition of PG synthesis was accompanied by a decrease in the concentration of sodium and mucus in the gastric juice but by an increase in the output and concentration of hydrogen ion. These changes suggest a possible mechanism by which endogenous PG play a role in the regulation of gastric secretion and in the protection against gastrointestinal damage.
前列腺素（PG）已知可改变多种胃肠功能，但内源性PG的生理作用仍不清楚。本实验旨在评估用消炎痛（5毫克/千克，皮下注射）急性和慢性抑制PG合成后胃液分泌的变化。在用生理盐水或消炎痛治疗1天或4天后，通过连续抽吸收集8只适应坐在椅子上的清醒雄性恒河猴的胃液。分析胃液中H +、Na +、K +和Cl -的浓度。使用阿尔辛蓝染料结合酸性糖蛋白和高碘酸希夫反应结合中性糖蛋白来估计胃液中可溶性黏液的量。测量血浆以及胃底、胃窦和十二指肠活检样本中的PG水平。消炎痛治疗1天和4天均显著（p < 0.05）降低了胃底、胃窦和十二指肠中的组织PG水平。消炎痛治疗1天和4天后，血浆中PGF2α水平均显著（p < 0.05）降低，而PGE2和6 - 酮 - PGF1α仅在消炎痛治疗4天后受到显著抑制。PG合成的急性和慢性抑制均伴随着胃液中钠和黏液浓度的降低，但氢离子输出量和浓度增加。这些变化提示了一种可能的机制，内源性PG通过该机制在胃液分泌调节和胃肠道损伤防护中发挥作用。
