Department of Restorative Dentistry, Endodontics Division, Piracicaba Dental School, State University of Campinas-UNICAMP, Piracicaba, SP, Brazil.
Department of Periodontology, Dental Research Division, Guarulhos University, Guarulhos, São Paulo, Brazil.
Clin Oral Investig. 2020 Sep;24(9):3243-3254. doi: 10.1007/s00784-020-03200-z. Epub 2020 Jan 20.
The objective of this study was to evaluate the presence of microorganisms in the root canals (RC) of teeth submitted to endodontic retreatment due to prosthetic reasons and without evidence of apical periodontitis.
Twenty teeth referred to endodontic retreatment due to prosthetic reasons and without evidence of apical periodontitis were included in this study. Gutta-percha (GP) was collected from each third of the RC. After GP removal, microbial samples were also collected from the full extension of RC using paper points and files. The samples were cultivated in Fastidious Anaerobe Agar and the colony-forming units (CFU/mL) were counted. They were also semi-quantitatively analyzed by checkerboard DNA-DNA hybridization using 40 DNA bacterial probes.
Microorganisms were found in all samples. The coronal third of GP was more contaminated than the apical third (p ≤ 0.05). There was prevalence of Enterococcus hirae and Enterococcus faecalis in all RC thirds and also in the samples collected from the full extension of RCs.
Microorganisms were found in all cases referred to endodontic retreatment due to prosthetic reasons and without evidence of apical periodontitis. Enterococcus was the genus most frequently detected.
An endodontic retreatment should be considered before replacing a prosthesis.
本研究旨在评估因修复原因而进行根管再治疗且无根尖周炎证据的牙齿根管（RC）中微生物的存在情况。
本研究纳入了 20 颗因修复原因而需要进行根管再治疗且无根尖周炎证据的牙齿。从 RC 的每三分之一处取出牙胶尖。在去除牙胶尖后，还使用纸尖和锉从 RC 的全长采集微生物样本。将样本接种于苛养厌氧菌琼脂，并计算每毫升的集落形成单位（CFU/mL）。还使用 40 个细菌 DNA 探针的棋盘式 DNA-DNA 杂交对半定量分析。
所有样本中均发现了微生物。牙胶尖的冠部三分之一比根尖部三分之一污染更严重（p≤0.05）。所有 RC 三分之一和 RC 全长采集的样本中均存在屎肠球菌和粪肠球菌。
因修复原因而进行根管再治疗且无根尖周炎证据的所有病例中均发现了微生物。肠球菌是最常检测到的属。
在更换义齿之前，应考虑进行根管再治疗。
