Ghasemi M, Parhizkar Roudsari P, Ghasem Ahangari M, Takzaree N
Department of Anatomy and Medicinal Plants Research Center, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Arch Razi Inst. 2024 Dec 31;79(6):1197-1205. doi: 10.32592/ARI.2024.79.6.1197. eCollection 2024 Dec.
The restoration of healthy human skin function in the early stages is contingent upon the effective healing of wounds. This process requires the highest quality of care. While previous research has examined the individual impacts of Mentha Piperita and clinoptilolite, this experiment demonstrates their synergistic effects, presenting a promising new approach to expedite wound recovery. The study employed a total of 60 male Wistar rats, which were randomly assigned to one of five experimental groups: a negative control group, a positive control group (phenytoin ointment), a clinoptilolite group, an M. Piperita group, and an M. Piperita + clinoptilolite group. All ethical considerations were upheld, and neck wounds were created. Histological examinations were conducted on days 4, 7, and 14. Additionally, collagen deposition, inflammation, and re-epithelialization scores were evaluated on day 14 using the scoring system developed by Abramov. Wound healing assays were conducted on days 4, 7, 10, and 14. A p-value of less than 0.05 was considered statistically significant. The M. Piperita + clinoptilolite group demonstrated a statistically significant increase in the number of fibroblasts and vessels, accompanied by a notable reduction in the inflammatory cell count, when compared to all other experimental groups within the wound site. Furthermore, all groups exhibited significantly elevated collagen deposition and re-epithelialization scores (P-value <0.05) and diminished inflammatory cell infiltration (though not reaching statistical significance) compared to the control group. Furthermore, the M. Piperita + clinoptilolite group exhibited a markedly reduced wound surface area and accelerated healing rate compared to all other groups on all experimental days. On day 14, the recovery percentage reached an impressive 99.75 ± 0.5. The combination of clinoptilolite and M. piperita exerts a significant influence on wound healing, as evidenced by the histopathologic parameters and clinical outcomes. This combination is more effective than the separate usage of either substance or phenytoin.
早期恢复健康的人体皮肤功能取决于伤口的有效愈合。这个过程需要最高质量的护理。虽然先前的研究已经考察了薄荷和斜发沸石的各自影响，但本实验证明了它们的协同作用，为加速伤口恢复提供了一种有前景的新方法。该研究总共使用了60只雄性Wistar大鼠，它们被随机分配到五个实验组之一：阴性对照组、阳性对照组（苯妥英软膏）、斜发沸石组、薄荷组和薄荷 + 斜发沸石组。所有伦理考量均得到遵守，并制造了颈部伤口。在第4天、第7天和第14天进行了组织学检查。此外，在第14天使用Abramov开发的评分系统评估了胶原蛋白沉积、炎症和再上皮化评分。在第4天、第7天、第10天和第14天进行了伤口愈合测定。p值小于0.05被认为具有统计学意义。与伤口部位的所有其他实验组相比，薄荷 + 斜发沸石组的成纤维细胞和血管数量在统计学上显著增加，同时炎症细胞计数显著减少。此外，与对照组相比，所有组的胶原蛋白沉积和再上皮化评分均显著升高（P值 <0.05），炎症细胞浸润减少（尽管未达到统计学意义）。此外，在所有实验天数中，薄荷 + 斜发沸石组的伤口表面积均明显减小，愈合速度加快。在第14天，恢复百分比达到了令人印象深刻的99.75 ± 0.5。组织病理学参数和临床结果表明，斜发沸石和薄荷的组合对伤口愈合有显著影响。这种组合比单独使用任何一种物质或苯妥英更有效。
