Guo Pengfei, Zhao Song, Han Xiaoli, Huang Jingtao, Liang Zongying
Department of Thoracic Surgery, Affiliated Hospital of Chengde Medical University, Chengde, China.
Hebei Key Laboratory of Panvascular Diseases, Chengde, China.
Cytotechnology. 2025 Aug;77(4):130. doi: 10.1007/s10616-025-00797-5. Epub 2025 Jun 29.
Previously, there have been reports of decreased expression of GRIM-19 and increased expression of MDM2 in lung adenocarcinoma. However, the relationship between GRIM-19 and MDM2 in lung adenocarcinoma has not been reported yet. In this study, we demonstrated that GRIM-19mRNA expression was reduced in lung adenocarcinoma tissue while MDM2mRNA expression was increased. Pearson correlation coefficient analysis showed a significant correlation between GRIM-19mRNA and MDM2mRNA (r = -0.970, < 0.001). In this study, we further detected by experiments that the overexpression of GRIM-19 lowered the expression level of MDM2 protein, suggesting that GRIM-19 may affect the occurrence and development of lung adenocarcinoma by inhibiting the expression of MDM2. Cellular functional and subcutaneous tumour formation experiments in nude mice have shown that GRIM-19 inhibits the proliferation, invasion, and migration ability of lung adenocarcinoma cells, promotes cell apoptosis, and ultimately inhibits lung adenocarcinoma development. In addition, overexpression of GRIM-19 in A549 cells resulted in significant changes in the expression of EMT-related proteins Snail, N-Cadherin, E-Cadherin, and Vimentin, indicating that GRIM-19 may inhibit the metastasis of lung adenocarcinoma cells by regulating the EMT pathway.
The online version contains supplementary material available at 10.1007/s10616-025-00797-5.
此前，有报道称在肺腺癌中GRIM-19表达降低而MDM2表达增加。然而，肺腺癌中GRIM-19与MDM2之间的关系尚未见报道。在本研究中，我们证明肺腺癌组织中GRIM-19mRNA表达降低而MDM2mRNA表达增加。Pearson相关系数分析显示GRIM-19mRNA与MDM2mRNA之间存在显著相关性（r = -0.970，<0.001）。在本研究中，我们通过实验进一步检测到GRIM-19的过表达降低了MDM2蛋白的表达水平，提示GRIM-19可能通过抑制MDM2的表达影响肺腺癌的发生发展。在裸鼠中的细胞功能和皮下肿瘤形成实验表明，GRIM-19抑制肺腺癌细胞的增殖、侵袭和迁移能力，促进细胞凋亡，最终抑制肺腺癌发展。此外，GRIM-19在A549细胞中的过表达导致EMT相关蛋白Snail、N-钙黏蛋白、E-钙黏蛋白和波形蛋白的表达发生显著变化，表明GRIM-19可能通过调节EMT途径抑制肺腺癌细胞的转移。
在线版本包含可在10.1007/s10616-025-00797-5获取的补充材料。
