Fatahi-Bafghi Mehdi
Research Center for Health Technology Assessment and Medical Informatics, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Int J Syst Evol Microbiol. 2025 Jul;75(7). doi: 10.1099/ijsem.0.006823.
In the current study, we evaluated the taxonomic relationship between and through a comprehensive comparison of phenotypic and genomic characteristics, as well as phylogenetic analysis. The 16S rRNA gene sequences of the type strains and shared a sequence similarity of 99.65%, which was above the threshold value (98.65%) for bacterial species delineation. In addition, whole-genome comparisons revealed that DSM 935 and DSM 2950 shared 97.06% average nucleotide identity by blast (ANIb), 98.38% average nucleotide identity using MUMmer (ANIm), 97.77% average amino acid identity and 84.80% digital DNA-DNA hybridization (dDDH) values. These scores exceed the threshold criteria for bacterial species delineation, strongly suggesting that these species belong to the same species. Furthermore, phylogenetic and phylogenomic analyses provided additional evidence that DSM 935 and DSM 2950 are very closely related to each other. Additionally, the phenotypic features of these species further revealed that they belong to the single species. On the basis of the results presented here and the principle of priority in nomenclature, (Kaneuchi . 1976) Liu . 2008 should be considered a later heterotypic synonym of (Prévot 1941) Liu . 2008.
在本研究中，我们通过对表型和基因组特征进行全面比较以及系统发育分析，评估了[具体物种1]和[具体物种2]之间的分类关系。模式菌株[具体物种1]和[具体物种2]的16S rRNA基因序列的序列相似性为99.65%，高于细菌物种划分的阈值（98.65%）。此外，全基因组比较显示，[具体物种1]DSM 935和[具体物种2]DSM 2950通过Blast（ANIb）的平均核苷酸同一性为97.06%，使用MUMmer（ANIm）的平均核苷酸同一性为98.38%，平均氨基酸同一性为97.77%，数字DNA-DNA杂交（dDDH）值为84.80%。这些分数超过了细菌物种划分的阈值标准，强烈表明这些物种属于同一物种。此外，系统发育和系统基因组分析提供了额外的证据，表明[具体物种1]DSM 935和[具体物种2]DSM 2950彼此密切相关。此外，这些物种的表型特征进一步表明它们属于单一物种。基于此处给出的结果和命名法中的优先权原则，[具体物种1]（金内uchi等人，1976年）刘等人，2008年应被视为[具体物种2]（普雷沃特，1941年）刘等人，2008年的晚出异名。
