Cavazzoli Chiara, Di Pasquale Roberto, Moghaddam Zarrin, Zhao Hongjuan, Hoxha Agron, Lewendon Lauren, Felipe-Sotelo Monica, Crean Carol, Zambon Alfonso, Lusvardi Gigliola, Merino-Gutierrez Jorge, Carta Daniela
School of Chemistry and Chemical Engineering, University of Surrey, GU2 7XH Guildford, UK.
Department of Chemical and Geological Sciences, University of Modena and Reggio Emilia, Modena, Italy.
J Mater Chem B. 2025 Jul 30;13(30):9153-9165. doi: 10.1039/d5tb00454c.
Phosphate-based glasses (PGs) are promising bioresorbable materials for controlled delivery of therapeutic species and tissue regeneration. The traditional method of synthesis of PGs involves the use of high temperatures, which limits their biomedical applications. The main goal of this work was to manufacture Mn loaded PGs for bone regeneration using an alternative, versatile and sustainable manufacturing technique. In this work, the novel room temperature, water-based method of coacervation was used for the synthesis of PGs in the system PO-CaO-NaO-(MnO) where = 0, 1, 3, 5, 10 mol% both in powder (PGPs) and fibre (PGFs) form. PGPs were manufactured by vacuum drying polyphosphate coacervate gels and PGFs by electrospinning them. The addition of Mn, which plays an important role in bone mineralization, represents a clear novelty of this work as Mn loaded PGs prepared coacervation have not been presented to date. Mn release in deionized (DI) water has been shown to increase with Mn loading in both PGPs and PGFs, demonstrating tailored release by modifying its content in the glass. biocompatibility was investigated for both systems MTT assay on human osteosarcoma cells (MG-63) at three different ratios of dissolution products to cell medium after 24 h immersion in DI water (1, 3 and 5% v/v). Results have demonstrated that PGPs and PGFs loaded with Mn up to 1 mol% are the most promising systems as they are not cytotoxic at all ratios investigated. Preliminary bioactivity tests performed by immersing a PGP sample containing 1 mol% of Mn in both cell medium (McCoy's 5A) and Tris-buffer solution for 24 and 72 h suggest the deposition of a disordered, possibly hydroxyapatite-like phase on the surface of the glass. This study demonstrates that PGPs and PGFs, synthesised coacervation, exhibit controlled release of the therapeutic ion Mn and promising biocompatibility, making them suitable candidates for applications such as bone regeneration and controlled delivery.
基于磷酸盐的玻璃（PGs）是用于治疗物质的可控释放和组织再生的有前景的生物可吸收材料。传统的PGs合成方法涉及高温使用，这限制了它们的生物医学应用。这项工作的主要目标是使用一种替代的、通用且可持续的制造技术来制造用于骨再生的载锰PGs。在这项工作中，采用了新颖的室温、水基凝聚法在PO-CaO-NaO-(MnO)体系中合成PGs，其中 = 0、1、3、5、10 mol%，制成粉末（PGPs）和纤维（PGFs）形式。PGPs通过真空干燥多磷酸盐凝聚凝胶制造，PGFs通过静电纺丝制造。在骨矿化中起重要作用的锰的添加是这项工作的一个明显新颖之处，因为迄今为止尚未报道过通过凝聚法制备的载锰PGs。已表明在去离子（DI）水中，PGPs和PGFs中的锰释放都随锰负载量增加，通过改变玻璃中的含量实现了定制释放。对两种体系的生物相容性进行了研究，在将溶解产物与细胞培养基以三种不同比例（1、3和5% v/v）在DI水中浸泡24小时后，对人骨肉瘤细胞（MG-63）进行MTT测定。结果表明，负载锰高达1 mol%的PGPs和PGFs是最有前景的体系，因为在所有研究比例下它们都没有细胞毒性。通过将含有1 mol%锰的PGP样品在细胞培养基（McCoy's 5A）和Tris缓冲溶液中浸泡24小时和72小时进行的初步生物活性测试表明，在玻璃表面沉积了一种无序的、可能类似羟基磷灰石的相。这项研究表明，通过凝聚法合成的PGPs和PGFs表现出治疗性离子锰的可控释放和有前景的生物相容性，使其成为骨再生和可控释放等应用的合适候选材料。
