Norwood Aria, Jamora Jadyn, Madison Micuel, Nguyen Jamiee, Yousaf Azan, Morales Katya, Vu Emily, Hernández-Gómez Obed
Department of Natural Sciences and Mathematics, Dominican University of California, San Rafael, California, USA.
Department of Fish, Wildlife and Conservation Ecology, New Mexico State University, Las Cruces, New Mexico, USA.
Environ Microbiol. 2025 Jul;27(7):e70131. doi: 10.1111/1462-2920.70131.
Microbial surveys are becoming an important component of wildlife health research, especially in cases where environmental change and infectious diseases are serious issues. Amongst amphibians, Batrachochytrium spp. have the potential to be fatal pathogens that can impact the population health of numerous species. We assessed the skin bacteriome, prevalence of Batrachochytrium dendrobatidis (Bd) and body conditions of two salamander genera with different life histories: the California slender salamander (Batrachoseps attenuatus) and members of the Pacific newt species complex (Taricha torosa and Taricha granulosa; Taricha sp.). We used 16S rRNA V4 amplicon sequencing to characterise the bacterial communities. Taricha sp. had higher prevalence and loads of Bd compared to B. attenuatus, although the prevalence of Bd was restricted to two of the four regions tested. Both salamander types possessed similar bacterial community richness, and the composition of the skin bacteriome varied mostly by region. However, we did find certain associations between the salamander genera and certain ASVs, with mostly members of the family Burkholderiaceae driving the difference. Our results provide additional evidence for the presence of structure in Bd prevalence and bacteriome composition amongst sympatric amphibians that inhabit different microhabitats.
微生物调查正成为野生动物健康研究的一个重要组成部分，尤其是在环境变化和传染病成为严重问题的情况下。在两栖动物中，蛙壶菌属有可能成为致命病原体，会影响众多物种的种群健康。我们评估了两种具有不同生活史的蝾螈属的皮肤微生物群落、蛙壶菌（Bd）的感染率和身体状况：加利福尼亚细趾蝾螈（Batrachoseps attenuatus）以及太平洋蝾螈物种复合体的成员（粗皮蝾螈Taricha torosa和加州蝾螈Taricha granulosa；Taricha sp.）。我们使用16S rRNA V4扩增子测序来表征细菌群落。与细趾蝾螈相比，Taricha sp.的Bd感染率和负荷更高，尽管Bd的感染率仅限于所测试的四个区域中的两个区域。两种蝾螈类型的细菌群落丰富度相似，并且皮肤微生物群落的组成主要因区域而异。然而，我们确实发现蝾螈属与某些ASV之间存在特定关联，主要是伯克霍尔德氏菌科的成员导致了这种差异。我们的结果为栖息在不同微生境中的同域两栖动物中Bd感染率和微生物群落组成的结构存在提供了更多证据。
