Kuyama J, Kanayama Y, Katagiri S, Tamaki T, Yonezawa T, Tarui S
Ultrastruct Pathol. 1985;8(2-3):155-63. doi: 10.3109/01913128509142149.
Tubuloreticular inclusions (TRI) and paired cisternae (PC) were induced in lymphocytes of normal individuals after incubation with Staphylococcus aureus Cowan 1. TRI were initially detected in lymphoid cells on day 2 (48-h culture). The frequency of TRI-positive cell sections on day 5 increased about twofold over those on days 2-4. On day 7, TRI were predominantly seen in lymphoplasmacytoid cells or plasmacytoid cells, with an incidence of up to 18% of sections. The regions in these cells were most extensive and anastomosed with the cisternae of adjacent well-developed rough endoplasmic reticulum (RER). TRI formation appears not to be essential for mitogen-induced B-cell differentiation to plasmacytoid cells, because pokeweed mitogen (PWM) failed to induce TRI. The diverse expressions of TRI induction between these two mitogens may be due to a difference in B-cell activation mechanisms. Paired cisternae were observed in a great majority of mitotic cells at various stages. These were encountered most frequently on day 4. PC were also seen in the PWM-stimulated culture. Our observations suggest that PC formation may be related to new formation of RER as well as to reconstruction of the nuclear envelope.
用金黄色葡萄球菌考恩1株孵育正常个体的淋巴细胞后，可诱导出管网状包涵体（TRI）和配对池（PC）。TRI最初在培养2天（48小时培养）时在淋巴细胞中被检测到。第5天TRI阳性细胞切片的频率比第2 - 4天增加了约两倍。第7天，TRI主要见于淋巴浆细胞样细胞或浆细胞样细胞，切片发生率高达18%。这些细胞中的区域最为广泛，并与相邻发育良好的粗面内质网（RER）池吻合。TRI的形成似乎不是丝裂原诱导B细胞分化为浆细胞样细胞所必需的，因为商陆丝裂原（PWM）未能诱导TRI。这两种丝裂原在TRI诱导方面的不同表达可能是由于B细胞激活机制的差异。在不同阶段的绝大多数有丝分裂细胞中都观察到了配对池。在第4天最常遇到。在PWM刺激的培养物中也可见到PC。我们的观察结果表明，PC的形成可能与RER的新形成以及核膜的重建有关。
