Akiyama Kazuki, Tsuchihashi Shuhei, Morimoto Yusuke V
Graduate School of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan.
Department of Physics and Information Technology, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan.
Genes Cells. 2025 Jul;30(4):e70037. doi: 10.1111/gtc.70037.
Retinal plays a key role in light absorption across prokaryotes and eukaryotes, both in rhodopsin and bacteriorhodopsin systems. The multicellular social amoeba Dictyostelium discoideum exhibits positive phototaxis. However, retinal binding proteins such as rhodopsin have not been found in the genome of Dictyostelium cells. Herein, we microscopically examined the effects of retinal on Dictyostelium cells. On adding all-trans-retinal to the medium, Dictyostelium cells retracted their pseudopodia and became rounded. This was unique to retinal among the tested vitamin A variants. Addition of all-trans-retinal at low concentrations did not cause cell rounding. However, it increased the frequency of cAMP signaling triggered during cell development. Results indicate that retinal acts on an unknown signaling pathway involving the cytoskeleton in Dictyostelium cells.
视黄醛在原核生物和真核生物的光吸收过程中起着关键作用，无论是在视紫红质还是细菌视紫红质系统中。多细胞社会性变形虫盘基网柄菌表现出正向光趋性。然而，在盘基网柄菌细胞的基因组中尚未发现视黄醛结合蛋白，如视紫红质。在此，我们通过显微镜检查了视黄醛对盘基网柄菌细胞的影响。向培养基中添加全反式视黄醛后，盘基网柄菌细胞缩回伪足并变圆。在所测试的维生素A变体中，这对视黄醛来说是独特的。低浓度添加全反式视黄醛不会导致细胞变圆。然而，它增加了细胞发育过程中触发的环磷酸腺苷（cAMP）信号传导的频率。结果表明，视黄醛作用于盘基网柄菌细胞中涉及细胞骨架的未知信号通路。
