Ide Hayato, Hayashida Yukihisa, Morimoto Yusuke V
Graduate School of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan.
Department of Physics and Information Technology, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan.
Front Cell Dev Biol. 2023 Jul 20;11:1237778. doi: 10.3389/fcell.2023.1237778. eCollection 2023.
The bacterial signaling molecule cyclic diguanosine monophosphate (c-di-GMP) is only synthesized and utilized by the cellular slime mold among eukaryotes. cells undergo a transition from a unicellular to a multicellular state, ultimately forming a stalk and spores. While is known to employ c-di-GMP to induce differentiation into stalk cells, there have been no reports of direct observation of c-di-GMP using fluorescent probes. In this study, we used a fluorescent probe used in bacteria to visualize its localization within multicellular bodies. Cytosolic c-di-GMP concentrations were significantly higher at the tip of the multicellular body during stalk formation.
细菌信号分子环二鸟苷单磷酸（c-di-GMP）仅在真核生物中的细胞黏菌中合成和利用。细胞经历从单细胞状态到多细胞状态的转变，最终形成柄和孢子。虽然已知利用c-di-GMP诱导分化为柄细胞，但尚无使用荧光探针直接观察c-di-GMP的报道。在本研究中，我们使用了一种用于细菌的荧光探针来可视化其在多细胞体内的定位。在柄形成过程中，多细胞体顶端的胞质c-di-GMP浓度显著更高。
