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流感病毒核糖核蛋白的电泳分离

Electrophoretic separation of influenza virus ribonucleoproteins.

作者信息

Rees P J, Dimmock N J

出版信息

J Gen Virol. 1981 Mar;53(Pt 1):125-32. doi: 10.1099/0022-1317-53-1-125.

DOI:10.1099/0022-1317-53-1-125
PMID:7276909
Abstract

A new procedure for the separation of influenza virus particle ribonucleoproteins (RNPs) by electrophoresis on a slab of polyacrylamide gel resolves five discrete bands. The largest is a triplet containing the three largest RNAs (1 to 3), the intermediate sized RNP is a doublet containing RNAs 5 and 6 and the others each contain a single RNA. In addition, each RNP is composed of NP protein and an amount of M which is independent of the size of the RNP. This suggests that some virus particle M is specifically associated with influenza ribonucleoprotein.

摘要

一种通过在聚丙烯酰胺凝胶平板上进行电泳来分离流感病毒颗粒核糖核蛋白(RNP)的新方法可分辨出五条离散的条带。最大的条带是一个三联体,包含三个最大的RNA(1至3),中等大小的RNP是一个包含RNA 5和6的双峰,其他每个都包含单个RNA。此外,每个RNP由NP蛋白和一定量的M组成,M的量与RNP的大小无关。这表明一些病毒颗粒M与流感核糖核蛋白特异性相关。

相似文献

1
Electrophoretic separation of influenza virus ribonucleoproteins.流感病毒核糖核蛋白的电泳分离
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2
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