Ioannou Georgia D, Christodoulou Olga, Sofroniou Andria, Ioannou Katerina A, Christou Atalanti, Stavrou Ioannis J, Kapnissi-Christodoulou Constantina P
Department of Chemistry, University of Cyprus, 1678 Nicosia, Cyprus.
Department of Life Sciences, European University Cyprus, 2404 Nicosia, Cyprus.
Food Chem. 2025 Nov 15;492(Pt 1):145275. doi: 10.1016/j.foodchem.2025.145275. Epub 2025 Jun 24.
The study valorizes prickly pear peels, traditionally considered agro-waste, as a source of phenolic antioxidants using an eco-friendly extraction method. Natural deep eutectic solvents (NaDESs) combined with ultrasound-assisted extraction were optimized via response surface methodology to maximize phenolic recovery (80 % v/v choline chloride-citric acid NaDES in water, 30 mL/g solvent-to-solid ratio, 16-min extraction time). HPLC analysis identified catechin, myricetin, rutin, and gallic acid. To improve extraction efficiency, macrocyclic additives (cyclodextrins, CDs, and, for the first time, cyclofructans, CFs) were incorporated into the extraction medium. A systematic comparison of natural and derivatized CDs was also performed to address gaps in the literature. Method greenness and NaDES recyclability were validated using AGREE metrics. Chemometric analysis enabled the classification of samples according to botanical and geographical origin based on phenolic profiles. These results demonstrate an efficient, sustainable strategy for the recovery of valuable compounds applicable to natural product authentication, traceability, and development.
该研究利用一种环保的提取方法，将传统上被视为农业废弃物的仙人掌果皮作为酚类抗氧化剂的来源进行了价值评估。通过响应面法对天然低共熔溶剂（NaDESs）与超声辅助提取相结合的方法进行了优化，以最大限度地提高酚类物质的回收率（80% v/v氯化胆碱 - 柠檬酸NaDES在水中，溶剂与固体比例为30 mL/g，提取时间为16分钟）。高效液相色谱分析鉴定出了儿茶素、杨梅素、芦丁和没食子酸。为了提高提取效率，将大环添加剂（环糊精，CDs，以及首次使用的环果糖，CFs）加入到提取介质中。还对天然和衍生化的CDs进行了系统比较，以弥补文献中的空白。使用AGREE指标验证了方法的绿色度和NaDES的可回收性。化学计量分析能够根据酚类物质的谱图对样品按植物和地理来源进行分类。这些结果证明了一种高效、可持续的策略，可用于回收适用于天然产物认证、可追溯性和开发的有价值化合物。
