Varga B, Horváth E, Zsolnai B
Acta Endocrinol (Copenh). 1985 Oct;110(2):271-5. doi: 10.1530/acta.0.1100271.
Oestrous rats were anaesthetized with pentobarbital, one of the femoral arteries and veins and one of the ovarian veins were cannulated, and a thin polyethylene cannula was fixed in the ovarian bursa. Five-min blood fractions were collected from the ovary for 50 min. Following the control fractions 15 mU of oxytocin, 15 mU of vasopressin or 50 microliter of 0.9% NaCl solution was given into the ovarian bursa over 10 min. Blood pressure and ovarian blood flow were continuously recorded. Progesterone (P) and oestradiol-17 beta (E2) were determined from the blood samples by RIA. Oxytocin did not alter the blood pressure, whereas the ovarian blood flow showed a short increasing tendency. Later, however, it started to decrease in parallel with the decrease in blood pressure owing to blood loss. The secretion of P and E2 remained unchanged. No changes in blood pressure were observed after vasopressin administration, although the ovarian blood flow quickly decreased in parallel with the secretion of P and E2. It is suggested that oxytocin has no direct effect on ovarian blood flow and hormone secretion in the rat. Vasopressin, however, is an effective vasoconstrictor in the rat ovary and may in this way reduce hormone synthesis.
将动情期大鼠用戊巴比妥麻醉，分别插管一条股动脉、一条股静脉和一条卵巢静脉，并在卵巢囊内固定一根细的聚乙烯插管。从卵巢收集5分钟的血样，共收集50分钟。在对照血样采集后，将15 mU的催产素、15 mU的加压素或50微升0.9%氯化钠溶液在10分钟内注入卵巢囊。持续记录血压和卵巢血流。通过放射免疫分析法从血样中测定孕酮（P）和雌二醇-17β（E2）。催产素未改变血压，而卵巢血流呈现短暂的增加趋势。然而，随后由于失血导致血压下降，卵巢血流也开始随之下降。P和E2的分泌保持不变。给予加压素后未观察到血压变化，尽管卵巢血流与P和E2的分泌同时迅速下降。提示催产素对大鼠卵巢血流和激素分泌无直接影响。然而，加压素是大鼠卵巢中的一种有效血管收缩剂，可能通过这种方式减少激素合成。
