Madkaiker Deep, Ojha Shashank, N Arunkumar, Chawan Kalpesh
Department of Transfusion Medicine, Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Homi Bhabha National Institute, Mumbai, India.
Department of Immunohematology and Blood Transfusion, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India.
Afr J Lab Med. 2025 Jun 25;14(1):2723. doi: 10.4102/ajlm.v14i1.2723. eCollection 2025.
Leukoreduction is a post-processing technique that reduces residual leukocytes in cellular blood components. Previous studies have evaluated these parameters mainly among older generation leuko-filters.
This study evaluated the immediate effects of pre-storage leukoreduction on red cell indices and the performance efficacy of two newer generation leuko-filters.
This retrospective analysis collected quality control data before and after leukoreduction for erythrocyte concentrates (ECs) from laboratory registers from the Blood Transfusion Laboratory at the Advanced Centre for Treatment, Research and Education in Cancer in Mumbai, India, for the period January 2015 to December 2019. Data related to red cell indices and performance characteristics for Fresenius and Macopharma filters were included.
A total of 500 records was included in the study. All EC units demonstrated a 99.99% leukocyte log reduction, with both filters showing equal efficacy. Post-leukoreduction haemoglobin concentrations were lower than the pre-leukoreduction for all units ( < 0.001). Of those prepared from 350 mL units, 11.6% (28/240) had haemoglobin levels under 40 g/bag as compared to 1.1% (3/260) among those prepared from 450 mL units. All indices exhibited statistically significant changes after leukoreduction ( < 0.001) except for mean corpuscular haemoglobin ( = 0.215). The Fresenius filter required less time for leukoreduction compared to the Macopharma filter ( < 0.001).
Red cell indices show several changes following leukoreduction. Further studies are needed to assess the microscopic and functional impact of leukoreduction. Leuko-filters vary in their performance characteristics, which may influence vendor selection.
This study found changes among several red cell indices after leukoreduction of ECs, which have not been extensively studied in previous literature. Further, we found that the newer generation leuko-filters differ in specific performance characteristics, which may influence vendor selection.
白细胞滤除是一种减少细胞血液成分中残留白细胞的后处理技术。以往研究主要在老一代白细胞滤器中评估这些参数。
本研究评估储存前白细胞滤除对红细胞指数的即时影响以及两种新一代白细胞滤器的性能功效。
本回顾性分析从印度孟买癌症治疗、研究与教育高级中心输血实验室的实验室登记册中收集了2015年1月至2019年12月期间浓缩红细胞（EC）白细胞滤除前后的质量控制数据。纳入了与费森尤斯和麦科菲姆滤器的红细胞指数及性能特征相关的数据。
本研究共纳入500条记录。所有EC单位的白细胞对数减少率均达99.99%，两种滤器显示出相同的功效。所有单位白细胞滤除后的血红蛋白浓度均低于滤除前（<0.001）。由350 mL单位制备的产品中，11.6%（28/240）的血红蛋白水平低于40 g/袋，而由450 mL单位制备的产品中这一比例为1.1%（3/260）。除平均血红蛋白含量外（P = 0.215），白细胞滤除后所有指数均呈现出具有统计学意义的变化（<0.001）。与麦科菲姆滤器相比，费森尤斯滤器进行白细胞滤除所需时间更短（<0.001）。
白细胞滤除后红细胞指数出现了若干变化。需要进一步研究来评估白细胞滤除的微观和功能影响。白细胞滤器的性能特征各不相同，这可能会影响供应商的选择。
本研究发现EC白细胞滤除后多个红细胞指数发生了变化，此前文献对此未进行广泛研究。此外，我们发现新一代白细胞滤器在特定性能特征方面存在差异，这可能会影响供应商的选择。
