Bai Yao, Ma Jiaqi, Li Fengqin, Yang Baowei, Ren Xiu, Wang Yeru, Hu Yujie, Dong Yinping, Wang Wei, Zhang Jing, Yan Shaofei, Cui Shenghui
NHC Key Laboratory of Food Safety Risk Assessment, China National Centre for Food Safety Risk Assessment, Beijing 100022, China.
College of Food Science and Engineering, Northwest Agriculture and Forestry Science and Technology University, Shaanxi 712100, China.
Microorganisms. 2024 Aug 6;12(8):1601. doi: 10.3390/microorganisms12081601.
Objective species are the main causes of foodborne illness worldwide, posing significant threats to public health. This study aimed to investigate the antibiotic resistance and genomic characterization of / from retail chickens in Beijing. Methods Antimicrobial susceptibility testing was conducted on 126 / isolated from retail chickens in Beijing, following CLSI protocols. Whole genomes of all isolates were sequenced using the Illumina platform. Results More (83.82%) showed multi-drug resistance than (8.62%). Genomic analysis demonstrated 42 sequence types (STs) and 12 clonal complexes (CCs), from which CC828 and CC52 were dominant. , and encoding cytotoxic protein were present spontaneously in most but not found in any isolates. The abundances of antibiotic resistance genes (ARGs) and virulence genes (VGs) in and were significantly different, with ARGs numbered in and VGs in . Conclusions High prevalence of multi-drug resistance and isolated from Beijing chickens were challenging clinical antibiotic usages in the treatment of infection. The surveillance of particular and STs correlated with higher resistance and virulence needs to be strengthened in the future.
目的 某些物种是全球食源性疾病的主要病因，对公众健康构成重大威胁。本研究旨在调查北京零售鸡肉中[具体物种]的抗生素耐药性及基因组特征。方法 按照美国临床和实验室标准协会（CLSI）的规程，对从北京零售鸡肉中分离出的126株[具体物种]进行药敏试验。使用Illumina平台对所有分离株的全基因组进行测序。结果 更多的[具体物种]（83.82%）表现出多重耐药性，而[另一物种]（8.62%）则较少。基因组分析显示有42种序列类型（STs）和12个克隆复合体（CCs），其中CC828和CC52占主导地位。编码细胞毒性蛋白的[具体基因1]、[具体基因2]和[具体基因3]在大多数[具体物种]中自发存在，但在任何[另一物种]分离株中均未发现。[具体物种]和[另一物种]中抗生素耐药基因（ARGs）和毒力基因（VGs）的丰度显著不同，[具体物种]中的ARGs数量为[具体数字]，VGs数量为[具体数字]。结论 从北京鸡肉中分离出的[具体物种]和[另一物种]多重耐药性的高流行率对临床治疗[具体感染类型]时抗生素的使用构成挑战。未来需要加强对与更高耐药性和毒力相关的特定[具体物种]和[另一物种]STs的监测。
