Ali Asmahan A, Aljumayi Huda, Aljutaily Thamer, Alfheeaid Hani A, Al-Zunaidy Nada Bint Abdullah, Mohamed Ahmed Isam A, Mohammed Belal M, Khalil Nazeha A
Department of Food Science and Human Nutrition, College of Agriculture and Food Qassim University Buraydah Saudi Arabia.
Department of Food Science and Nutrition, College of Sciences Taif University Taif Saudi Arabia.
Food Sci Nutr. 2025 Jul 7;13(7):e70556. doi: 10.1002/fsn3.70556. eCollection 2025 Jul.
Kaempferol is a natural flavonoid abundant in many plant-based foods; eatable fruits and vegetables including broccoli. It has been used for its potential health benefits, including hypolipidemic properties and a wide range of pharmacological activities as antioxidants contributing to numerous diseases treatment. The current study aimed to measure kaempferol between microwaved broccoli (MB) samples using high-performance liquid chromatography (HPLC). Also, their potential hypolipidemic properties between hyperlipidemic rats were investigated: MB at different times (1, 2 and 5 min) fed randomly rat groups by 2.5% each with two control groups positive vs. negative, and collected blood samples were used for blood glucose (BG) and lipid profiles cholesterol; CHO, triglycerides; TG, high-density lipoprotein-cholesterol (HDL-c), and low-density lipoprotein-cholesterol (LDL-c) and again for kidney (Urea, Criatinine) and liver (alanine & aspartate aminotransferase) functions with histological analysis. Collected data showed kaempferol levels only in two MB samples (2 & 5 min; about 56 and 68 mg/100 g, respectively). While the animals' body weight levels declined with different improvements in lipids, kidney, and liver functions. The lowest BG was seen with rats fed a 2 min microwaved cooking period (104.33 ± 1.15 mg/dL) that was close to control rats (102.67 ± 2.08 mg/dL). Also, HDL-c increased and LDL-c decreased while the biggest shown within rats fed 1 min samples (33.80 ± 2.83 mg/dL). Overall, kaempferol in MB demonstrated potential in modulating levels of BG, lipids, kidney, and liver functions between hyperlipidemic models; thus, incorporating MB at 2-5 min influenced the antioxidant levels, providing a valuable adjunct to traditional lowering lipid therapy.
山奈酚是一种天然黄酮类化合物，在许多植物性食物中含量丰富，包括可食用的水果和蔬菜，如西兰花。它因其潜在的健康益处而被使用，包括降血脂特性以及作为抗氧化剂的广泛药理活性，有助于治疗多种疾病。本研究旨在使用高效液相色谱法（HPLC）测量微波处理西兰花（MB）样品中的山奈酚含量。此外，还研究了它们在高脂血症大鼠中的潜在降血脂特性：将不同时间（1、2和5分钟）的MB以2.5%的比例随机喂食大鼠组，设有两个对照组，即阳性对照组和阴性对照组，并采集血样用于检测血糖（BG）和血脂谱，包括胆固醇（CHO）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-c）和低密度脂蛋白胆固醇（LDL-c），还再次用于检测肾脏（尿素、肌酐）和肝脏（丙氨酸和天冬氨酸转氨酶）功能，并进行组织学分析。收集的数据显示，仅在两个MB样品（2分钟和5分钟；分别约为56和68毫克/100克）中检测到山奈酚水平。而动物的体重水平随着脂质、肾脏和肝脏功能的不同改善而下降。喂食2分钟微波烹饪时间的大鼠血糖最低（104.33±1.15毫克/分升），接近对照组大鼠（102.67±2.08毫克/分升）。此外，HDL-c升高，LDL-c降低，其中喂食1分钟样品的大鼠中变化最为明显（33.80±2.83毫克/分升）。总体而言，MB中的山奈酚在调节高脂血症模型中的BG、脂质、肾脏和肝脏功能水平方面显示出潜力；因此，2 - 5分钟的MB摄入会影响抗氧化剂水平，为传统的降脂治疗提供了有价值的辅助手段。
