Al-Haliem Siba Mouid, Mohammed Muthanna Jasim, Abedelmaksoud Tarek Gamal, Hesarinejad Mohammad Ali, Baioumy Ahmed Adel
Department of Dental Basic Sciences College of Dentistry, University of Mosul Mosul Iraq.
Department of Biology, College of Education for Pure Sciences University of Mosul Mosul Iraq.
Food Sci Nutr. 2025 Jun 30;13(7):e70550. doi: 10.1002/fsn3.70550. eCollection 2025 Jul.
, a plant with a long history of medicinal use, was extracted using three solvents: hexane, ethanol, and ethyl acetate. The resulting extracts were subjected to chromatographic analysis, yielding five distinct fractions. Fractions I and II were obtained from the ethyl acetate extract, whereas Fractions III, IV, and V were derived from the ethanol extract. This study evaluated the antibacterial activity of these fractions against five virulent Gram-negative bacteria associated with periodontitis: , , , , and . The results demonstrated that Fraction I exhibited significant antibacterial activity, with an inhibition zone of 26 mm against and at a concentration of 200 μg/mL. Fraction II showed notable inhibition against and . Fraction III achieved a maximum inhibition zone of 35 mm against at 100 μg/mL and at 200 μg/mL. Similarly, Fraction IV exhibited potent antibacterial activity, with a peak inhibition zone of 35 mm at 100 μg/mL. Fraction V also produced an inhibition zone of 35 mm against at 100 μg/mL. Compared with standard antibiotics, such as ampicillin and cephalosporin, the fractions demonstrated comparable efficacy. In addition to antibacterial activity, the study investigated the antioxidant and free radical scavenging capacities of the isolated fractions at various concentrations (ppm), using vitamin C as a reference. Fractions I and II showed the highest antioxidant activity at 30 ppm, with values reaching 22.36. Fraction V exhibited the most potent activity, with antioxidant values of 35.65 at 30 ppm, 56.22 at 60 ppm, 76.44 at 120 ppm, and continued enhancement at 250 ppm. These findings highlight the promising bioactive potential of extracts and provide a solid foundation for further investigation into their therapeutic applications in managing bacterial infections and oxidative stress. The detailed results emphasize the importance of continued research into the mechanisms and complexities of these bioactive compounds.
一种有着悠久药用历史的植物，使用三种溶剂进行提取：己烷、乙醇和乙酸乙酯。对所得提取物进行色谱分析，得到五个不同的馏分。馏分I和II来自乙酸乙酯提取物，而馏分III、IV和V来自乙醇提取物。本研究评估了这些馏分对与牙周炎相关的五种致病性革兰氏阴性菌的抗菌活性：[具体细菌名称1]、[具体细菌名称2]、[具体细菌名称3]、[具体细菌名称4]和[具体细菌名称5]。结果表明，馏分I表现出显著的抗菌活性，在浓度为200μg/mL时，对[具体细菌名称1]和[具体细菌名称2]的抑菌圈为26mm。馏分II对[具体细菌名称3]和[具体细菌名称4]表现出显著抑制作用。馏分III在100μg/mL时对[具体细菌名称5]的最大抑菌圈为35mm，在200μg/mL时对[具体细菌名称6]的最大抑菌圈为35mm。同样，馏分IV表现出强大的抗菌活性，在100μg/mL时峰值抑菌圈为35mm。馏分V在100μg/mL时对[具体细菌名称7]的抑菌圈也为35mm。与氨苄青霉素和头孢菌素等标准抗生素相比，这些馏分显示出相当的疗效。除抗菌活性外，该研究还以维生素C为参照，研究了不同浓度（ppm）下分离出的馏分的抗氧化和自由基清除能力。馏分I和II在30ppm时表现出最高的抗氧化活性，值达到22.36。馏分V表现出最强的活性，在30ppm时抗氧化值为35.65，在60ppm时为56.22，在120ppm时为76.44，并在250ppm时持续增强。这些发现突出了[植物名称]提取物具有的有前景的生物活性潜力，并为进一步研究其在管理细菌感染和氧化应激方面的治疗应用提供了坚实基础。详细结果强调了持续研究这些生物活性化合物的机制和复杂性的重要性。
