Vargas Diana, Merle Roswitha, Friese Anika, Roesler Uwe, Robé Caroline
Veterinary Centre for Resistance Research, Institute of Animal Hygiene and Environmental Health, Freie Universität Berlin, Berlin, Germany.
Veterinary Centre for Resistance Research, Institute of Veterinary Epidemiology and Biostatistics, Freie Universität Berlin, Berlin, Germany.
Sci Rep. 2025 Jul 8;15(1):24547. doi: 10.1038/s41598-025-10279-5.
Antimicrobial-resistant bacteria originating from broilers pose an ongoing challenge as they can spread in the environment and food chain. One approach to lower colonization is to administer live bacteria to broilers. A live Escherichia coli (E. coli) vaccine, consisting of a single E. coli strain used as a competitive exclusion (CE) approach, was evaluated to decrease the colonization with ESBL-/pAmpC- producing E. coli. 168 ESBL-/pAmpC- negative Ranger Gold broilers were divided into six groups (3 x n = 46 experimental groups; 3 x n = 10 control groups for quality assurance). The E. coli vaccine was administered on day one through coarse spray or by drinking water on day five. Experimental groups were orally co-colonized with 10 cfu of one ESBL- (ST410, bla) and one pAmpC- producing E. coli (ST10, bla/mcr-1) on day three. Colonization status was monitored throughout the trial and quantified at the end of the study (day 49). A transient reduction in ESBL-producing E. coli colonization (p < 0.001) was observed following coarse spray administration. However, this decrease was not sustained over time. It can be concluded that a single E. coli strain originating from a live vaccine cannot decrease colonization of broilers with ESBL-/pAmpC- E. coli throughout a fattening period.
源自肉鸡的抗菌药物耐药性细菌是一个持续存在的挑战，因为它们可在环境和食物链中传播。降低定植的一种方法是给肉鸡施用活菌。评估了一种由单一大肠杆菌菌株组成的活大肠杆菌疫苗，作为一种竞争排除（CE）方法，以减少产ESBL-/pAmpC的大肠杆菌的定植。168只ESBL-/pAmpC阴性的兰杰黄金肉鸡被分为六组（3×n = 46个实验组；3×n = 10个用于质量保证的对照组）。大肠杆菌疫苗在第1天通过粗喷雾或在第5天通过饮水施用。实验组在第3天口服共同定植10 cfu的一种产ESBL（ST410，bla）和一种产pAmpC的大肠杆菌（ST10，bla/mcr-1）。在整个试验过程中监测定植状态，并在研究结束时（第49天）进行量化。粗喷雾施用后观察到产ESBL大肠杆菌定植的短暂减少（p < 0.001）。然而，这种减少并未随时间持续。可以得出结论，源自活疫苗的单一大肠杆菌菌株在整个育肥期内不能减少肉鸡被ESBL-/pAmpC大肠杆菌定植的情况。
