Ju Cheng-Wei, Lyu Ruitu, Li Han, Wei Jiangbo, Parra Vitela Alberto J, Dougherty Urszula, Kwesi Akushika, Luna Alexander, Zhu Xuanhao, Shen Shenghai, Liu Yunzheng, Wang Liangliang, Cui Xiaolong, Xu Yuzhi, Jiang Bochen, Ji Yiyi, Xia Peng, West-Szymanski Diana C, Sun Chenxi, Zhong Yuhao, Ye Chang, Moran Angelica, Lehmann Christopher, Pamer Eric, Zhang Wei, Bissonnette Marc, Zhang Li-Sheng, He Chuan
Department of Chemistry, The University of Chicago, Chicago, IL, USA.
Howard Hughes Medical Institute, The University of Chicago, Chicago, IL, USA.
Nat Biotechnol. 2025 Jul 8. doi: 10.1038/s41587-025-02731-8.
Circulating cell-free RNA (cfRNA) in plasma represents a promising avenue for cancer detection. We report low-input multiple methylation sequencing, a method for profiling modification patterns in cfRNA, enabling the detection of diverse transfer RNAs and small noncoding RNAs derived from both the human genome and the microbiome. RNA modification patterns in microbiome-derived cfRNA accurately reflect host microbiota activity and hold potential for the early detection of colorectal cancer.
血浆中的循环游离RNA（cfRNA）是癌症检测的一个有前景的途径。我们报告了低输入量多重甲基化测序，这是一种用于分析cfRNA修饰模式的方法，能够检测源自人类基因组和微生物组的多种转运RNA和小非编码RNA。微生物组衍生的cfRNA中的RNA修饰模式准确反映宿主微生物群的活性，并具有早期检测结直肠癌的潜力。
