Wang Zhaoqi, Xie Changping, Lin Xuetuan, Zheng Li, Chen Daipeng
National Key Laboratory for Tropical Crop Breeding, School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya, Hainan, China.
Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, School of Tropical Agriculture and Forestry, Ministry of Education, Hainan University, Haikou, Hainan, China.
Arch Virol. 2025 Jul 8;170(8):174. doi: 10.1007/s00705-025-06362-x.
A previously uncharacterized mitovirus, designated as "Diaporthe mitovirus 3" (DMV3), was identified and isolated from the fungal pathogen Diaporthe sp. (isolate B-16) in this study. The DMV3 genome is a positive-sense, single-stranded RNA (+ ssRNA) molecule of 2,319 nucleotides (nt) with a GC content of 40%. It contains a single open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp) consisting of 679 amino acids (aa) with a molecular mass of 76.4 kDa. Comparative analysis using BLASTp revealed that the RdRp of DMV3 exhibits the highest sequence similarity (64.56% identity) to Pestalotiopsis mitovirus 2 (PMV2). Phylogenetic analysis based on RdRp sequences demonstrated that DMV3 clusters together with PMV2 within a distinct clade, supporting its classification as a member of the genus Unuamitovirus within the family Mitoviridae.
在本研究中，从真菌病原体Diaporthe sp.（分离株B - 16）中鉴定并分离出一种以前未被表征的线粒体病毒，命名为“Diaporthe mitovirus 3”（DMV3）。DMV3基因组是一个2319个核苷酸（nt）的正义单链RNA（+ ssRNA）分子，GC含量为40%。它包含一个单一的开放阅读框（ORF），编码一个由679个氨基酸（aa）组成的RNA依赖性RNA聚合酶（RdRp），分子量为76.4 kDa。使用BLASTp进行的比较分析表明，DMV3的RdRp与拟盘多毛孢线粒体病毒2（PMV2）表现出最高的序列相似性（64.56%的同一性）。基于RdRp序列的系统发育分析表明，DMV3与PMV2在一个独特的进化枝中聚集在一起，支持将其分类为线粒体病毒科中Unuamitovirus属的成员。
