Li Bai, Liu Xiaoying, Zang Jinping, Cao Hongzhe, Si Helong, Zhang Kang, Xing Jihong, Dong Jingao
State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding, China.
Hebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding, China.
Front Microbiol. 2025 Jun 24;16:1595008. doi: 10.3389/fmicb.2025.1595008. eCollection 2025.
Kynurenine monooxygenase, a vital rate-limiting enzyme in the kynurenine pathway metabolic branch, has shown promise as a drug target for treating human neurodegenerative diseases. However, the role of kynurenine monooxygenase in plant pathogens and its potential as a molecular target have received limited attention. In this study, we identified a novel kynurenine monooxygenase gene, , in . By generating mutants of this gene, it was found that the deletion of affected the changes of key metabolites in the kynurenine pathway , and the △ mutant exhibits reduced growth and fails to produce sclerotia. Additionally, changes were observed in the morphology of mycelium cells and spores, and the mutant's pathogenicity was weakened. These findings indicate that positively regulates the growth, development, and pathogenic processes of . Furthermore, we screened two antibacterial peptides, CAMPQ3966 and CAMPQ4589, that target using MEGADOCK, HDOCK, and AlphaFold3. Both peptides effectively inhibited the pathogenicity of . These findings provide the foundation for developing novel drug targets for controlling gray mold.
犬尿氨酸单加氧酶是犬尿氨酸途径代谢分支中的一种重要限速酶，已显示出作为治疗人类神经退行性疾病药物靶点的潜力。然而，犬尿氨酸单加氧酶在植物病原体中的作用及其作为分子靶点的潜力受到的关注有限。在本研究中，我们在[具体物种]中鉴定出一个新的犬尿氨酸单加氧酶基因[基因名称]。通过产生该基因的突变体，发现[基因名称]的缺失影响了犬尿氨酸途径中关键代谢物的变化，并且△突变体生长减缓且不能产生菌核。此外，观察到菌丝细胞和孢子的形态发生了变化，并且突变体的致病性减弱。这些发现表明[基因名称]正向调节[具体物种]的生长、发育和致病过程。此外，我们使用MEGADOCK、HDOCK和AlphaFold3筛选了两种靶向[基因名称]的抗菌肽CAMPQ3966和CAMPQ4589。两种肽均有效抑制了[具体物种]的致病性。这些发现为开发控制灰霉病的新型药物靶点奠定了基础。
