两种培养技术的比较：体外和体内肿瘤集落形成试验。
A comparison of two culture techniques: an in vitro & an in vivo tumour colony-forming assay.
作者信息
Slee P H, Willemze R, van Oosterom A T, Lurvink E, van den Berg L
出版信息
Br J Cancer. 1985 Nov;52(5):713-7. doi: 10.1038/bjc.1985.248.
Twenty-one identical tumour specimens were cultured both in the Plasma-Clot Diffusion Chamber (PCDC) Technique and the Human Tumour Colony-forming Assay (HTCA). The culture results achieved in the PCDC-technique were clearly superior to the HTCA: in the PCDC the mean and median plating efficiency (PE) was 0.156 and 0.147, in the HTCA 0.103 and 0.028%; adequate growth rate in the PCDC-technique was 67% and in the HTCA 38%. Fewer cells were required for plating in the PCDC-technique: 6.4 X 10(4) vs. 2.6 X 10(5) in the HTCA. The mean and median coefficient of variation of the colony numbers in the PCDC-technique appeared much higher: 27.3 and 37.3 vs. 11.2 and 11.1% in the HTCA. The relation between the PEs obtained for the same specimen in the two techniques was compared. No positive correlation was found, which can possibly be ascribed to technical shortcomings in both techniques.
21个相同的肿瘤标本分别采用血浆凝块扩散室（PCDC）技术和人肿瘤集落形成试验（HTCA）进行培养。PCDC技术的培养结果明显优于HTCA：PCDC技术中平均接种效率（PE）和中位接种效率分别为0.156和0.147，HTCA中分别为0.103和0.028%；PCDC技术中合适的生长率为67%，HTCA中为38%。PCDC技术接种所需的细胞数更少：分别为6.4×10⁴个细胞，而HTCA中为2.6×10⁵个细胞。PCDC技术中集落数的平均变异系数和中位变异系数明显更高：分别为27.3和37.3，而HTCA中分别为11.2和11.1%。比较了两种技术对同一样本获得的接种效率之间的关系。未发现正相关，这可能归因于两种技术的技术缺陷。
Zotero 插件