酶免疫测定法检测乙型肝炎表面抗原

Enzyme-immunoassay in the detection of hepatitis B surface antigen.

作者信息

Ukkonen P, Koistinen V, Penttinen K

出版信息

J Immunol Methods. 1977;15(4):343-53. doi: 10.1016/0022-1759(77)90096-5.

Abstract

A new enzyme-immunoassay (EIA, Hepanostika Microelisa System) for the detection of hepatitis B surface antigen was evaluated against other methods, namely complement fixation, Hepanosticon, AusRIA II and Finnish Red Cross Radioimmunoassay (FRC-RIA). EIA detected the greatest number of positive samples in a serum panel consisting of 142 sera from clinical hepatitis patients. FRC-RIA was the most sensitive method for subtype ad, while EIA detected the ay specimen at the highest dilution. None of the test systems gave the 'optimal' result in the screening test, and it is proposed that a separate procedure for each antigen subtype should be carried out to detect the greatest number of positive samples.

摘要

一种用于检测乙型肝炎表面抗原的新型酶免疫测定法（EIA，Hepanostika Microelisa系统）与其他方法进行了比较，这些方法包括补体结合试验、Hepanosticon、AusRIA II和芬兰红十字会放射免疫测定法（FRC-RIA）。在一组由142份临床肝炎患者血清组成的血清样本中，EIA检测出的阳性样本数量最多。FRC-RIA是检测ad亚型最灵敏的方法，而EIA能在最高稀释度下检测出ay样本。在筛查试验中，没有一种检测系统能给出“最佳”结果，建议针对每种抗原亚型采用单独的检测程序，以检测出最多的阳性样本。