Cai Xinghua, Xu Yuanying, Gao Jie, Zhang Huihui, Lu Jun, Lei Tao
Shanghai Putuo Central School of Clinical Medicine, Anhui Medical University, Shanghai, China.
The Fifth School of Clinical Medicine, Anhui Medical University, Hefei, China.
J Diabetes Res. 2025 Jul 2;2025:2540444. doi: 10.1155/jdr/2540444. eCollection 2025.
The objective of the study is to determine the association between thyroid function parameters and plasma levels of branched-chain amino acids (BCAAs) and their metabolites, branched-chain keto acids (BCKAs), in patients with euthyroid Type 2 diabetes mellitus (T2DM). We conducted a cross-sectional study among 357 participants with euthyroid T2DM. Plasma BCAAs and BCKAs were measured using enzyme-linked immunosorbent assay (ELISA), while four thyroid function parameters, including triiodothyronine (T3), thyroxine (T4), free triiodothyronine (FT3), and free thyroxine (FT4), were determined by an automatic biochemical analyzer. The variance inflation factor (VIF) was used to investigate variable collinearity. Pearson correlation and multivariate linear regression analysis were used to establish the relationship between BCAAs or BCKAs and thyroid function parameters. The means of T3, T4, FT3, and FT4 were different among the three groups divided by BCAA tertiles (all < 0.05), and the means of T3 and FT3 were different among the three groups divided by BCKA tertiles (all < 0.01). Pearson correlation analysis indicated a positive relationship between all the thyroid function parameters and BCAA levels ( < 0.05), while a statistically significant inverse relationship was established between T3 and FT3 levels and BCKA levels ( < 0.01). Collinearity analysis demonstrated no collinearity among the variables (tolerance: 0.5010.922; VIF: 1.0841.995). Multivariate linear regression analysis showed that thyroid function parameters were significantly associated with BCAAs (coefficients: 0.042 to 4.614, < 0.05) and BCKAs (coefficients: -0.200 to -0.156, < 0.01) after adjusting for other potential confounding factors. Thyroid function parameters positively correlated with plasma BCAAs but negatively correlated with plasma BCKAs in patients with euthyroid T2DM. However, further prospective studies are needed to explore potential causal relationships and verify these findings.
本研究的目的是确定甲状腺功能参数与甲状腺功能正常的2型糖尿病（T2DM）患者血浆中支链氨基酸（BCAAs）及其代谢产物支链酮酸（BCKAs）水平之间的关联。我们对357名甲状腺功能正常的T2DM参与者进行了一项横断面研究。采用酶联免疫吸附测定（ELISA）法测定血浆BCAAs和BCKAs，同时用自动生化分析仪测定包括三碘甲状腺原氨酸（T3）、甲状腺素（T4）、游离三碘甲状腺原氨酸（FT3）和游离甲状腺素（FT4）在内的四项甲状腺功能参数。用方差膨胀因子（VIF）研究变量共线性。采用Pearson相关性分析和多元线性回归分析来建立BCAAs或BCKAs与甲状腺功能参数之间的关系。按BCAA三分位数分组的三组中，T3、T4、FT3和FT4的均值存在差异（均P<0.05），按BCKA三分位数分组的三组中，T3和FT3的均值存在差异（均P<0.01）。Pearson相关性分析表明，所有甲状腺功能参数与BCAA水平呈正相关（P<0.05），而T3和FT3水平与BCKA水平呈统计学显著负相关（P<0.01）。共线性分析表明变量之间不存在共线性（容忍度：0.5010.922；VIF：1.0841.995）。多元线性回归分析显示，在调整其他潜在混杂因素后，甲状腺功能参数与BCAAs（系数：0.042至4.614，P<0.05）和BCKAs（系数：-0.200至-0.156，P<0.01）显著相关。在甲状腺功能正常的T2DM患者中，甲状腺功能参数与血浆BCAAs呈正相关，但与血浆BCKAs呈负相关。然而，需要进一步的前瞻性研究来探索潜在的因果关系并验证这些发现。
