Das Saurav Shankar, Krishnamurthy Nandini N, Kumar Mohana, Dsouza Riolla Sanchia
Department of Periodontics and Oral Implantology, A.J. Institute of Dental Sciences, Mangalore, Karnataka, India.
Nitte University Centre for Stem Cell Research and Regenerative Medicine, Mangalore, Karnataka, India.
J Indian Soc Periodontol. 2025 Jan-Feb;29(1):63-72. doi: 10.4103/jisp.jisp_266_24. Epub 2025 Jun 10.
Initial research suggested a potential link between titanium particles and peri-implantitis. Titanium's susceptibility to fretting, a process that generates microscopic debris, and its passive layer's breakdown in inflammatory environments contribute to the release of titanium ions. However, the full impact of these particles on implant health remains unclear. Therefore, the aim of the study is to evaluate the effect of different concentrations of titanium nanoparticles on the cytotoxicity and matrix metalloproteinase-8 expression in human periodontal ligament fibroblasts (HPDLfs).
To investigate the effects of titanium (Ti) on HPDLfs, a standard Ti solution was diluted to create various concentrations (0.001, 0.1, 1, and 100 ppm). HPDLFs were cultured in media containing these Ti concentrations, and their viability was assessed after 24 and 48 h. Based on these cytotoxicity results, optimal Ti concentrations (0.01, 0.1, and 10 ppm) were selected for further analysis. The expression of matrix metalloproteinase-8 (MMP-8) was measured using quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) to evaluate the impact of Ti on HPDLfs.
Exposure to titanium (Ti) reduced the viability of HPDLFs in a concentration-and time-dependent manner. At 100 ppm, more than 50% cell death was observed. qRT-PCR indicated a significant up-regulation of MMP-8 expression. However, ELISA analysis did not reveal any significant changes in MMP-8 levels.
Within the study's limitations, the cytotoxic and immunological responses of HPDLFs to titanium (Ti) are directly linked to the concentration and duration of exposure. Although no significant differences in MMP-8 release were observed across varying TiO2 concentrations, a decrease in cell viability was associated with higher TiO2 levels.
初步研究表明钛颗粒与种植体周围炎之间可能存在联系。钛易发生微动磨损，这一过程会产生微观碎片，且其钝化层在炎症环境中会分解，从而导致钛离子释放。然而，这些颗粒对种植体健康的全面影响仍不明确。因此，本研究的目的是评估不同浓度的钛纳米颗粒对人牙周膜成纤维细胞（HPDLfs）的细胞毒性和基质金属蛋白酶 - 8表达的影响。
为研究钛（Ti）对HPDLfs的影响，将标准Ti溶液稀释以产生不同浓度（0.001、0.1、1和100 ppm）。HPDLFs在含有这些Ti浓度的培养基中培养，并在24小时和48小时后评估其活力。基于这些细胞毒性结果，选择最佳Ti浓度（0.01、0.1和10 ppm）进行进一步分析。使用定量实时聚合酶链反应（qRT-PCR）和酶联免疫吸附测定（ELISA）测量基质金属蛋白酶 - 8（MMP - 8）的表达，以评估Ti对HPDLfs的影响。
暴露于钛（Ti）会以浓度和时间依赖性方式降低HPDLFs的活力。在100 ppm时，观察到超过50%的细胞死亡。qRT-PCR表明MMP - 8表达显著上调。然而，ELISA分析未发现MMP - 8水平有任何显著变化。
在本研究的局限性范围内，HPDLFs对钛（Ti）的细胞毒性和免疫反应与暴露的浓度和持续时间直接相关。尽管在不同TiO2浓度下未观察到MMP - 8释放的显著差异，但细胞活力的降低与较高的TiO2水平相关。
