Pourhaji Mohadeese, Abbasi Farid, Sehatpour Aliyeh, Bakhtiari Ronak
Department of Oral Medicine, School of Dentistry, Shahed University, Tehran, Iran.
School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Galen Med J. 2023 Dec 17;12:e2905. doi: 10.31661/gmj.v12i.2905. eCollection 2023.
The applications of nanotechnology have greatly increased in the recent years. Nanotechnology can be used for diagnosis and treatment of many conditions in medicine and dentistry. The aim of this paper is assessment the cytotoxicity of silver nanoparticles (AGNPs) synthesized employing Lactobacillus acidophilus against human oral squamous cell carcinoma (OSCC) cell line.
In this in vitro, experimental study, AgNPs were biologically synthesized by using L. acidophilus, and characterized by dynamic light scattering (DLS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), ultraviolet-visible (UV-V) spectroscopy and Fourier-transform infrared (FTIR) spectroscopy. The methyl thiazolyl tetrazolium (MTT) test was performed to assess the cytotoxic effects of AgNPs in 3.125, 6.25, 12.5, 25, 50, and 100 μg/mL concentrations within 24 hours.
Synthesis of AgNPs was confirmed by visual perception of dark brown color variation (from achromatic) and maximum UV-V absorption at 428 nm. TEM and SEM indicated the spherical form of AgNPs with a median size of 397 nm. FTIR spectroscopy showed the presence of functional groups from the cells involved in the reduction process. The MTT assay indicated that the biosynthesized nanoparticles made a decrease of cell livability in a concentration dependent method.
AgNPs produced by Lactobacillus acidophilus have the potential to inhibit OSCC cell line.
近年来，纳米技术的应用大幅增加。纳米技术可用于医学和牙科领域多种病症的诊断和治疗。本文旨在评估利用嗜酸乳杆菌合成的银纳米颗粒（AGNPs）对人口腔鳞状细胞癌（OSCC）细胞系的细胞毒性。
在这项体外实验研究中，通过嗜酸乳杆菌生物合成AgNPs，并采用动态光散射（DLS）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）、紫外可见（UV-V）光谱和傅里叶变换红外（FTIR）光谱对其进行表征。进行甲基噻唑基四氮唑（MTT）试验，以评估24小时内浓度为3.125、6.25、12.5、25、50和100μg/mL的AgNPs的细胞毒性作用。
通过肉眼观察到颜色从无色变为深棕色以及在428nm处的最大UV-V吸收，证实了AgNPs的合成。TEM和SEM显示AgNPs呈球形，中位尺寸为397nm。FTIR光谱表明参与还原过程的细胞中存在官能团。MTT试验表明，生物合成的纳米颗粒以浓度依赖性方式降低细胞活力。
嗜酸乳杆菌产生的AgNPs具有抑制OSCC细胞系的潜力。
