Lü Jia, Li You, Liu Shunmin, Younas Muhammad, Lu Lianming, Pu Zhanxu, Zhu Li, Chen Guoqing, Huang Zhendong
The Citrus Research Institute of Zhejiang Province/Key Lab of Fruit and Vegetable Function and Health Research of Taizhou, Zhejiang Academy of Agricultural Sciences, Taizhou, Zhejiang, China.
State Key Lab of Agricultural and Forestry Biosecurity, College of Plant Protection, Fujian Agriculture and Forestry Univ, Fuzhou, Fujian, China.
Neotrop Entomol. 2025 Jul 10;54(1):83. doi: 10.1007/s13744-025-01299-x.
Aphids (Hemiptera: Aphididae) are well-known agricultural pests, affecting global crop production both through direct feeding and as the vector of multiple plant viruses. The classification of citrus aphid species was outdated and limited within the Zhejiang Province, China. A comprehensive investigation was conducted to determine the aphid species associated with citrus cultivars from 2019 to 2024. The present study included an analysis of occurrence rate, ecological contexts, and microscope slides photographs of apterous viviparous female, along with the key morphometric parameters such as body length, antenna, rostrum, hind tarsus, cauda, and siphunculi. Our morphological and molecular data revealed five species in this particular citrus region. Among the identified species, Aphis (Aphis) gossypii Glover and A. (A.) spiraecola Patch were the most prevalent, followed by A. (A.) craccivora Koch, A. (Toxoptera) citricidus (Kirkaldy), and A. (T.) aurantii Boyer de Fonscolombe. Furthermore, the genetic variation among aphid clades was assessed using Kimura 2-parameter (K2P) model. The results showed that citrus aphid species exhibits low intraspecific genetic variation and high interspecific genetic variation. Although the morphometric parameters of these aphid species are relatively similar, they can be distinguished through extensive morphological characteristics and cytochrome c oxidase subunit I (COI) fragment. Additionally, this study also addresses the historical and current distinctions among A. (A.) spiraecola and A. (T.) citricidus. These findings will significantly contribute to the ongoing research and management of citrus aphids in citrus plantations.
蚜虫（半翅目：蚜科）是著名的农业害虫，通过直接取食和作为多种植物病毒的传播媒介影响全球作物生产。在中国浙江省，柑橘蚜虫种类的分类已经过时且有限。2019年至2024年进行了一项全面调查，以确定与柑橘品种相关的蚜虫种类。本研究包括对无翅胎生雌蚜的发生率、生态环境和显微镜载玻片照片进行分析，以及对体长、触角、喙、后跗节、尾片和腹管等关键形态测量参数进行分析。我们的形态学和分子数据揭示了这个特定柑橘种植区有五个物种。在已鉴定的物种中，棉蚜Aphis (Aphis) gossypii Glover和绣线菊蚜A. (A.) spiraecola Patch最为常见，其次是豆蚜A. (A.) craccivora Koch、桔蚜A. (Toxoptera) citricidus (Kirkaldy)和橘二叉蚜A. (T.) aurantii Boyer de Fonscolombe。此外，使用Kimura 2-参数（K2P）模型评估了蚜虫类群之间的遗传变异。结果表明，柑橘蚜虫种类表现出低种内遗传变异和高种间遗传变异。虽然这些蚜虫种类的形态测量参数相对相似，但可以通过广泛的形态特征和细胞色素c氧化酶亚基I（COI）片段来区分。此外，本研究还探讨了绣线菊蚜和桔蚜之间的历史和当前差异。这些发现将对柑橘种植园中柑橘蚜虫的持续研究和管理做出重大贡献。
