Mathioudakis Matthaios M, Varikou Kyriaki, Karagianni Antonia, Psirofonia Panagiota, Tektonidis Nikolaos, Kapantaidaki Despoina, Evangelou Vasiliki, Economou Leonidas, Hasiów-Jaroszewska Beata, Potamitis Ilyas
Plant Pathology Laboratory, Institute of Olive Tree, Subtropical Crops and Viticulture, ELGO-DIMITRA, Karamanlis Ave. 167, 73134 Chania, Crete Island, Greece.
Department of Entomology, Institute of Olive Tree, Subtropical Crops and Viticulture, ELGO-DIMITRA, Karamanlis Ave. 167, 73134 Chania, Crete Island, Greece.
Viruses. 2025 Mar 11;17(3):395. doi: 10.3390/v17030395.
(CTV) is a vector-borne virus that poses a significant threat to citrus production worldwide, inducing a variety of symptoms. Therefore, a detailed knowledge of local aphids, identification of viruliferous species, and the development of new monitoring tools are necessary to improve CTV control strategies. Herein, a 2-year survey was conducted to assess the frequency of aphid species infesting several citrus pilot orchards. Plot findings based on morphological and molecular identification revealed (ranged from 44-100%) as the most abundant aphid species, followed by (<50%). , and were present in low numbers, and was not detected. Due to the absence of CTV detection in aphids and citrus trees from the pilot orchards, a complementary survey was conducted in CTV-infected fields. Three aphid species were identified as CTV-positive by RT-PCR, suggesting that they may be viruliferous, with as predominant, followed by and . Additionally, we developed a non-invasive procedure for identifying aphid species using wingbeat analysis. This method provides a faster alternative to traditional identification techniques by taxonomic keys based on morphological features or PCR, although its accuracy is lower (approximately 95% for the two species tested). Overall, this work provides a detailed study of aphid species composition in citrus orchards, identifies the predominant local putative CTV vector, and introduces a novel sensor for aphid monitoring, contributing to improved epidemic forecasting and sustainable disease management strategies.
柑橘衰退病毒（CTV）是一种通过媒介传播的病毒，对全球柑橘生产构成重大威胁，会引发多种症状。因此，详细了解当地蚜虫、鉴定带毒种类以及开发新的监测工具对于改进CTV防控策略十分必要。在此，我们进行了为期两年的调查，以评估侵染多个柑橘试验果园的蚜虫种类的频率。基于形态学和分子鉴定的调查结果显示，[具体蚜虫种类1]（占比44%-100%）是最常见的蚜虫种类，其次是[具体蚜虫种类2]（占比<50%）。[具体蚜虫种类3]和[具体蚜虫种类4]数量较少，未检测到[具体蚜虫种类5]。由于在试验果园的蚜虫和柑橘树中未检测到CTV，我们在CTV感染的果园进行了补充调查。通过逆转录聚合酶链反应（RT-PCR）鉴定出三种蚜虫为CTV阳性，表明它们可能携带病毒，其中[具体蚜虫种类1]为主，其次是[具体蚜虫种类2]和[具体蚜虫种类3]。此外，我们开发了一种利用翅振分析鉴定蚜虫种类的非侵入性方法。该方法为基于形态特征或PCR的传统分类鉴定技术提供了一种更快的替代方法，尽管其准确性较低（测试的两种蚜虫的准确率约为95%）。总体而言，这项工作对柑橘园蚜虫种类组成进行了详细研究，确定了当地主要的假定CTV传播媒介，并引入了一种新型蚜虫监测传感器，有助于改进疫情预测和可持续病害管理策略。
