Pronobis Mira I
Department of Biological Sciences, University of Denver, Denver, CO, USA.
Knoebel Institute for Healthy Aging, University of Denver, Denver, CO, USA.
Methods Mol Biol. 2025;2953:205-214. doi: 10.1007/978-1-0716-4694-6_13.
Proximity labeling is a powerful technique for mapping protein networks in their native biological context, allowing for the precise identification of protein interactomes. Although widely used in cell culture systems, its application to in vivo studies remains limited. This protocol outlines the establishment of in vivo proximity labeling in adult zebrafish, a highly versatile vertebrate model known for its remarkable regenerative capacity and genetic tractability.
邻近标记是一种在天然生物学背景下绘制蛋白质网络的强大技术，可实现蛋白质相互作用组的精确鉴定。尽管该技术在细胞培养系统中被广泛应用，但其在体内研究中的应用仍然有限。本方案概述了在成年斑马鱼中建立体内邻近标记的方法，斑马鱼是一种高度通用的脊椎动物模型，以其卓越的再生能力和遗传易处理性而闻名。
