Ghobadi Sina, Shakib Pegah, Dirbaziyan Ashkan, Zargar Mohsen
Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran.
Assistant Professor of Medical Bacteriology, Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Recent Pat Biotechnol. 2025 Jul 9. doi: 10.2174/0118722083366860250623142119.
Aeromonas hydrophila, a rod-shaped, gram-negative bacterium, is frequently found in aquatic surroundings and additionally present in drinking water, sewage, and food sources. This microbe is gaining recognition as a potential threat to health, classifying it as an emerging pathogen. Biosurfactants are microbial-derived compounds that share hydrophilic and hydrophobic moieties that are surface active. This study aimed to investigate the effect of biosurfactant isolated from Actinobacteria on the expression of the bfp gene of A. hydrophila isolated from the stool samples of children referred to Hazrat Masoumeh Hospital in Qom, Iran, from May 2022 to March 2023.
Actinobacteria were isolated from soil samples of the desert areas of Qom province, Iran. Biochemical and molecular tests of 16S rRNA were used to identify Actinobacteria isolates. The produced biosurfactant was investigated by methods of hemolysis, oil droplet destruction, lipase production, oil expansion, emulsifying activity, and surface tension reduction measurement. The structure of biosurfactant was investigated by Fourier transform infrared spectroscopy (FTIR) analysis, and its effect on bfp gene expression was measured. Also, isolates of A. hydrophila were obtained from stool samples of children referred to Hazrat Masoumeh Hospital in Qom from May 2022 to March 2023. Then, the effect of a biosurfactant isolated from Actinobacteria on the bfp gene expression of A. hydrophila isolates was measured by RT-PCR.
Based on sequencing data, the Streptomyces genus with the ability to produce biosurfactant was isolated from the soil of the studied area, which could reduce the expression of the bfp gene after treatment with biosurfactant in clinical isolates of A. hydrophila.
The findings of the present study show that the desert soils of Qom province are a potential area for finding actinobacterial isolates with the ability to produce biosurfactants and influence the expression of pathogenic genes of clinical isolates of A. hydrophila.
嗜水气单胞菌是一种杆状革兰氏阴性菌，常见于水生环境中，也存在于饮用水、污水和食物来源中。这种微生物正逐渐被认为是对健康的潜在威胁，被归类为新兴病原体。生物表面活性剂是微生物衍生的化合物，具有亲水和疏水部分，具有表面活性。本研究旨在调查从放线菌中分离出的生物表面活性剂对2022年5月至2023年3月转诊至伊朗库姆哈扎特·马苏梅医院儿童粪便样本中分离出的嗜水气单胞菌bfp基因表达的影响。
从伊朗库姆省沙漠地区的土壤样本中分离放线菌。使用16S rRNA的生化和分子测试来鉴定放线菌分离株。通过溶血、油滴破坏、脂肪酶产生、油扩展、乳化活性和表面张力降低测量等方法研究产生的生物表面活性剂。通过傅里叶变换红外光谱（FTIR）分析研究生物表面活性剂的结构，并测量其对bfp基因表达的影响。此外，从2022年5月至2023年3月转诊至库姆哈扎特·马苏梅医院儿童的粪便样本中获得嗜水气单胞菌分离株。然后，通过RT-PCR测量从放线菌中分离出的生物表面活性剂对嗜水气单胞菌分离株bfp基因表达的影响。
根据测序数据，从研究区域的土壤中分离出具有产生生物表面活性剂能力的链霉菌属，在用生物表面活性剂处理后，其可降低嗜水气单胞菌临床分离株中bfp基因的表达。
本研究结果表明，库姆省的沙漠土壤是寻找具有产生生物表面活性剂能力并影响嗜水气单胞菌临床分离株致病基因表达的放线菌分离株的潜在区域。
