Salih Harith M, Amachawadi Raghavendra G, Kang Qing, Smolensky Dmitriy, Perumal Ramasamy, Bowser Sarah-Sexton, Prasad P V Vara, Nagaraja T G
Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS, United States.
Department of Statistics, College of Arts and Sciences, Kansas State University, Manhattan, KS, United States.
Front Cell Infect Microbiol. 2025 Jun 26;15:1568504. doi: 10.3389/fcimb.2025.1568504. eCollection 2025.
Liver abscesses that occur in finishing cattle fed high-grain, low-roughage diets, are of significant economic concern to the feedlot industry. The causative agents include both subspecies ( and ), , and serotype Lubbock. Tylosin, a macrolide antibiotic, is supplemented in the feed to reduce liver abscesses. Because of the concern with emergence of potential antimicrobial resistance, there is a need to find antibiotic alternatives. The aim of our study was to investigate the efficacy of phenolic compounds extracted from black and brown sumac sorghum extracts on liver abscess causing bacterial pathogens.
Phenolic compounds were extracted by 75% aqueous acetone and total phenolic content was determined spectrophotometrically. Muller-Hinton broth (for and ), and anaerobic Brain-Heart infusion broth (for ) with and without sorghum extracts (1 mg GAE/mL) were used. Growth was measured at 24 and 48 hours to determine bacterial concentration. Micro-broth dilution method was used to quantify growth inhibition.
Plant based phenolic compounds have the potential to be an antibiotic alternative to control liver abscesses. Sorghum [ (L.) Moench] grain phenolic compounds, have the potential to be one of these alternatives.
Our study demonstrated that the phenolic extracts of black and brown sumac sorghum exhibited antibacterial activities against the liver abscesses causing pathogens including both subspecies of and in a dose dependent manner, but not . Sorghum phenolic compounds have the potential to be supplemented in the cattle feed to control liver abscesses.
在育肥牛中，采食高谷物、低粗饲料日粮时发生的肝脓肿对育肥行业具有重大经济影响。病原体包括两个亚种（和）、、以及血清型拉伯克。泰乐菌素是一种大环内酯类抗生素，添加在饲料中以减少肝脓肿。由于担心潜在的抗菌药物耐药性出现，需要寻找抗生素替代品。我们研究的目的是调查从黑高粱和棕高粱提取物中提取的酚类化合物对引起肝脓肿的细菌病原体的功效。
用75%的丙酮水溶液提取酚类化合物，并通过分光光度法测定总酚含量。使用添加和不添加高粱提取物（1mg没食子酸当量/mL）的Muller-Hinton肉汤（用于和）以及厌氧脑心浸液肉汤（用于）。在24小时和48小时测量生长情况以确定细菌浓度。采用微量肉汤稀释法量化生长抑制情况。
基于植物来源的酚类化合物有可能成为控制肝脓肿的抗生素替代品。高粱[（L.）Moench]籽粒酚类化合物有可能成为这些替代品之一。
我们的研究表明，黑高粱和棕高粱的酚类提取物对包括和两个亚种在内的引起肝脓肿的病原体均表现出抗菌活性，且呈剂量依赖性，但对无此作用。高粱酚类化合物有可能添加到牛饲料中以控制肝脓肿。
