Yin Fan-Qian, Wang Xia-Yan, Li Yong-Xuan, Li Kai-Jing, Ma Si-Yu, Lv Meng-Jiao, Liu Zhen-Hua, Zhong Wei-Xia, Liu Yan, Tang Chuan-Fang, Liu Hong-Shi, Li Yuze, Xiao Fu-Hui, Kong Qing-Peng
State Key Laboratory of Genetic Evolution and Animal Models, State Key Laboratory of Genetic Resources and Evolution, Key Laboratory of Healthy Aging Research of Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650201, China.
Kunming College of Life Science, University of Chinese Academy of Sciences, Beijing 100049, China.
Proc Natl Acad Sci U S A. 2025 Jul 15;122(28):e2423142122. doi: 10.1073/pnas.2423142122. Epub 2025 Jul 11.
Identifying aging-associated biomarkers applicable for multiple tissues is challenging but crucial for assessing tissue aging. Here, we obtained and analyzed 456 transcriptomes on 17 organs from 30 C57BL/6 J mice with different ages, revealing the consistently upregulated mRNAs of , , and in most aged organs. This finding received support from independent transcriptomic and proteomic datasets and was further validated through western blot, enzyme-linked immunosorbent assay (ELISA), and immunofluorescence, arguing for both mRNA and protein as tissue-level aging biomarkers, at least in mice. Its sensitivity to antiaging interventions further emphasizes the significance of in assessing tissue aging in mice.
识别适用于多种组织的衰老相关生物标志物具有挑战性,但对于评估组织衰老至关重要。在此,我们获取并分析了来自30只不同年龄的C57BL/6 J小鼠17个器官的456个转录组,发现在大多数衰老器官中, 、 和 的mRNA持续上调。这一发现得到了独立转录组和蛋白质组数据集的支持,并通过蛋白质免疫印迹、酶联免疫吸附测定(ELISA)和免疫荧光进一步验证,表明 mRNA和蛋白质均为组织水平的衰老生物标志物,至少在小鼠中如此。其对抗衰老干预的敏感性进一步强调了 在评估小鼠组织衰老中的重要性。