Gul Anadil, Ahmad Munir, Ali Asghar, Boateng Derrick, Yang Liping, Kang Yan, Liao Wenchao
College of Health Science and Environmental Engineering, Shenzhen Technology University, Pingshan District, Shenzhen 518118, China; Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Shenzhen University Medical School, Shenzhen 518060, China.
Shenzhen Key Laboratory of Advanced Thin Films and Applications, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong, Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.
Colloids Surf B Biointerfaces. 2025 Nov;255:114935. doi: 10.1016/j.colsurfb.2025.114935. Epub 2025 Jul 5.
Osmium(bpy) complex coordinated with metal-organic framework UiO-67-bpy, photodynamic effect was studied in detail against pathogenic bacteria, under Near-IR (800-810 nm) irradiation. Structural analysis, including XRD, FTIR, and XPS, confirmed successful synthesis of the composite, highlighting Os-N ligation characterized by distinct peak at 623 cm with a slight red shift, and electronic modifications of N in XPS. Os(bpy)/UiO-67-bpy (UOsCb) composite demonstrated significantly enhanced photodynamic antibacterial efficacy, with 10.4 % bacterial viability (89.6 % inhibition) against Gram (+) S. aureus after just 5 min of irradiation. However, against Gram (-) E.coli the % viability was 33.4 % (66.6 % inhibition). Mechanistic studies revealed that UOsCb induced significantly higher 2.7 fold ROS (OH) generation compared to the bare Os-complex (1.37 fold) and UiO-67-bpy (1.2 fold), which play crucial role in photodynamic antibacterial efficacy. In vivo, UOsCb under Near-IR light significantly accelerated wound healing in S. aureus-infected models, achieving 90 % recovery within six days, compared to dark (45 %). Cytotoxicity (14.6 % over 48 h) and hemolysis (1.6 %) assays confirmed its excellent biocompatibility even at high concentrations (128 μM). This Near-IR-activated Type-I PSs offers promising alternative for antibacterial therapy, and novel platform for developing advanced heterojunction materials for biomedical applications.
锇（联吡啶）配合物与金属有机框架UiO-67-联吡啶配位，在近红外（800-810 nm）照射下，对致病细菌的光动力效应进行了详细研究。包括XRD、FTIR和XPS在内的结构分析证实了复合材料的成功合成，突出了Os-N键合，其特征是在623 cm处有明显峰值且有轻微红移，以及XPS中N的电子修饰。Os(bpy)/UiO-67-联吡啶（UOsCb）复合材料表现出显著增强的光动力抗菌效果，照射仅5分钟后，对革兰氏阳性金黄色葡萄球菌的细菌活力为10.4%（抑制率为89.6%）。然而，对革兰氏阴性大肠杆菌的活力百分比为33.4%（抑制率为66.6%）。机理研究表明，与裸露的Os配合物（1.37倍）和UiO-67-联吡啶（1.2倍）相比，UOsCb诱导产生的ROS（OH）显著高出2.7倍，这在光动力抗菌效果中起关键作用。在体内，近红外光下的UOsCb显著加速了金黄色葡萄球菌感染模型中的伤口愈合，与黑暗条件下（45%）相比，在六天内实现了90%的恢复。细胞毒性（48小时内为14.6%）和溶血（1.6%）试验证实了其即使在高浓度（128μM）下也具有优异的生物相容性。这种近红外激活的I型光动力剂为抗菌治疗提供了有前景的替代方案，也是开发用于生物医学应用的先进异质结材料的新型平台。
