Fu Xue, Tang Xun, Zhang Ning, Ren Xinyu, Li Peishan, Si Huaijun
State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China; College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.
State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China; College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
Plant Sci. 2025 Oct;359:112655. doi: 10.1016/j.plantsci.2025.112655. Epub 2025 Jul 9.
Potato is an important food and vegetable crop. Along with global climate change and the deepening industrialization of human life, drought and salinity have become important factors threatening potato production. The ubiquitin proteasome system is an important post-translational modification of proteins in eukaryotic organisms and plays an important role in plant resistance to abiotic and biotic stresses. In this study, StUBC19 was cloned from Atlantic and analyzed for tissue expression specificity and molecular characterization. Tissue specificity and molecular characterization were carried out and StUBC19 expression levels were significantly higher in leaves and terminal buds than in stem. histochemical staining showed that β-glucuronidase (GUS) activity driven by the StUBC19 promoter was induced by a variety of abiotic stresses (salt and drought) and phytohormones (abscisic acid, gibberellin, auxin, salicylic acid), which suggests that StUBC19 may be involved in a variety of signal transduction processes. StUBC19 may be involved in multiple signal transduction processes. The results of subcellular localization showed that StUBC19 was localized in the nucleus and plasma membrane. Transgenic plants overexpressing StUBC19 (OEs) showed increased plant height, root length, and biomass accumulation compared to wild-type plant (WT) under drought stress and salt stress. The transgenic lines had higher activities of antioxidant enzymes (superoxide dismutase, catalase, and peroxidase) and lower malondialdehyde levels. Yeast two-hybrid, bimolecular fluorescence complementation and luciferase complementation experiments confirmed the existence of a mutualistic relationship between StUBC19 and StWIN2. In conclusion, our results provide important reference information for the future use of StUBC19 in potato resistance breeding.
马铃薯是一种重要的粮食和蔬菜作物。随着全球气候变化以及人类生活工业化的深入，干旱和盐碱化已成为威胁马铃薯生产的重要因素。泛素蛋白酶体系统是真核生物中蛋白质重要的翻译后修饰途径，在植物抵抗非生物和生物胁迫中发挥重要作用。本研究从大西洋品种中克隆了StUBC19，并对其进行组织表达特异性和分子特征分析。开展了组织特异性和分子特征分析，结果表明StUBC19在叶片和顶芽中的表达水平显著高于茎。组织化学染色显示，由StUBC19启动子驱动的β-葡萄糖醛酸酶（GUS）活性受到多种非生物胁迫（盐和干旱）和植物激素（脱落酸、赤霉素、生长素、水杨酸）的诱导，这表明StUBC19可能参与多种信号转导过程。StUBC19可能参与多个信号转导过程。亚细胞定位结果表明，StUBC19定位于细胞核和质膜。与野生型植株（WT）相比，在干旱胁迫和盐胁迫下，过表达StUBC19的转基因植株（OEs）株高、根长和生物量积累增加。转基因株系具有更高的抗氧化酶（超氧化物歧化酶、过氧化氢酶和过氧化物酶）活性和更低的丙二醛水平。酵母双杂交、双分子荧光互补和荧光素酶互补实验证实了StUBC19与StWIN2之间存在相互作用关系。总之，我们的研究结果为未来StUBC19在马铃薯抗性育种中的应用提供了重要参考信息。
