Zheng Huiwen, Xu Zhipu, Fan Yiwen, Han Jiazhi, Zhou Liyang, Li Han, Pan Xiaohua, Ma Rongrong, Liu Chang, Tian Yaoqi
State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China.
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Foods. 2025 Jun 24;14(13):2217. doi: 10.3390/foods14132217.
Most studies on resistant starch are limited to its effect on blood glucose; there are few studies on the prebiotic effects of resistant starch on the gut. In this experiment, through in vivo metabolism verification and in vitro simulated fermentation experiments, it was found that hydroxypropyl oxide (OHS) had a prebiotic effect on the intestine. The results of bioinformatics showed that the structure of the microbiota changed significantly, and the in vitro and in vivo fermentation results of and showed an upward trend. The results of a KEGG prediction of the metabolic pathway showed that Phenylalanine metabolism and Cysteine and methionine metabolism showed an enhanced trend. At the same time, the results of in vitro and in vivo metabolite assays further confirmed this point, and the content of L-Homocystine and Phenylalanine in metabolites decreased significantly, with the decrease in L-Homocystine posing a reduction in cardiovascular disease risk and the decrease in Phenylalanine having a positive significance for phenylketonuria patients. This study proved that hydroxypropyl oxide can regulate the intestinal microbiota and has intestinal prebiotic effects, which can be used to guide the development of functional foods.
大多数关于抗性淀粉的研究仅限于其对血糖的影响；关于抗性淀粉对肠道的益生元效应的研究很少。在本实验中，通过体内代谢验证和体外模拟发酵实验，发现氧化羟丙基（OHS）对肠道具有益生元效应。生物信息学结果表明，微生物群的结构发生了显著变化，[具体物质]的体外和体内发酵结果呈上升趋势。代谢途径的KEGG预测结果表明，苯丙氨酸代谢以及半胱氨酸和甲硫氨酸代谢呈增强趋势。同时，体外和体内代谢物测定结果进一步证实了这一点，代谢物中L-同型半胱氨酸和苯丙氨酸的含量显著降低，L-同型半胱氨酸的降低降低了心血管疾病风险，苯丙氨酸的降低对苯丙酮尿症患者具有积极意义。本研究证明氧化羟丙基可以调节肠道微生物群并具有肠道益生元效应，可用于指导功能性食品的开发。
