Chufitskiy Sergey, Romanchuk Sergey, Meskhi Besarion, Olshevskaya Anastasiya, Shevchenko Victoria, Odabashyan Mary, Teplyakova Svetlana, Vershinina Anna, Savenkov Dmitry
Faculty of Biology, Donetsk State University, 24 Universitetskaya St., Donetsk 83001, Russia.
Agribusiness Faculty, Don State Technical University, Rostov-on-Don 344000, Russia.
Plants (Basel). 2025 Jul 1;14(13):2014. doi: 10.3390/plants14132014.
This study presents a biomonitoring study of surface waters in the Krynka River basin, encompassing three major regional reservoirs: Khanzhenkovskoe, Olkhovskoe, and Zuyevskoe. These water bodies face significant anthropogenic pressure from mining effluents, industrial discharges, and domestic wastewater. Key pollutants identified are surfactants (SAAs), sulfates, phenols, chlorides, and manganese, with chemical oxygen demand (COD) exceeding regulatory limits. The research was conducted in September 2024. Based on the Specific Combinatorial Water Pollution Index, surface waters in the studied objects can be characterized as slightly polluted. To assess the negative impact of the identified pollutants on hydrobionts, the species composition of phytoplankton of the studied water bodies was analyzed. In the Olkhovskoe Reservoir and Olkhovaya River, cyanobacterial blooms ( G.) were observed, altering biodiversity in the Krynka River and Zuyevskoe Reservoir. Phytoplankton genera , , and -established bioindicators of aquatic ecosystem health-were dominant in Khanzhenkovskoe Reservoir, signaling nutrient enrichment and organic pollution. Changes in the species composition and structure of phytoplankton in the Krynka River, its tributaries and reservoirs, indicate a change in the level of saprobic water bodies from β to α-mesosaprobic, which indicates both the general level of surface water pollution and the accumulation of pollutants along the course of the river. The paper presents the results of fluorimetric analysis of photosynthetic activity of natural phytoplankton cells and demonstrates the possibility of using fluorescence induction curves for regular monitoring measurements. Fluorescence parameters indicate a general deterioration of photosynthetic activity of natural phytoplankton. The growth of in the waters of the Olkhovskoe Reservoir and of green microalgae in the Zuevskoe Reservoir led to an increase in the fluorescence quantum yield (F/F) and the total photosynthetic activity index (PI), which makes it possible to use these parameters as indicator parameters reflecting the intensity of "blooming" of various phytoplankton species.
本研究对克林卡河流域的地表水进行了生物监测，该流域包括三个主要的区域水库：汉热恩科夫斯科耶水库、奥尔霍夫斯科耶水库和祖耶夫斯科耶水库。这些水体面临着来自采矿废水、工业排放和生活污水的巨大人为压力。已确定的主要污染物有表面活性剂（SAA）、硫酸盐、酚类、氯化物和锰，化学需氧量（COD）超过监管限值。该研究于2024年9月进行。根据特定组合水污染指数，研究对象中的地表水可被表征为轻度污染。为评估已确定污染物对水生生物的负面影响，分析了研究水体中浮游植物的物种组成。在奥尔霍夫斯科耶水库和奥尔霍瓦河中，观察到蓝藻水华（G.），这改变了克林卡河和祖耶夫斯科耶水库的生物多样性。汉热恩科夫斯科耶水库中浮游植物属、和（已确定的水生生态系统健康生物指标）占主导地位，表明存在营养物质富集和有机污染。克林卡河及其支流和水库中浮游植物的物种组成和结构变化表明，腐生性水体的水平从β中腐生性变为α中腐生性，这既表明了地表水的总体污染水平，也表明了污染物在河流沿线的积累。本文展示了天然浮游植物细胞光合活性的荧光分析结果，并证明了使用荧光诱导曲线进行定期监测测量的可能性。荧光参数表明天然浮游植物的光合活性普遍下降。奥尔霍夫斯科耶水库水体中（某种藻类）的生长以及祖耶夫斯科耶水库中绿色微藻的生长导致荧光量子产率（F/F）和总光合活性指数（PI）增加，这使得可以将这些参数用作反映各种浮游植物物种“水华”强度的指标参数。
