Malovrh Katja, Ravnjak Blanka, Križman Mitja, Bavcon Jože
Biotechnical Faculty, University of Ljubljana, University Botanic Gardens Ljubljana, Ižanska 15, 1000 Ljubljana, Slovenia.
Department of Analytical Chemistry, National Institute of Chemistry, Hajdrihova 19, 1001 Ljubljana, Slovenia.
Plants (Basel). 2025 Jul 6;14(13):2064. doi: 10.3390/plants14132064.
Jacq. and L. are common spring-flowering species in Slovenia. They occur in large stands and sometimes overgrow in unmanaged meadows. They are known as an excellent source of nectar for bees and other pollinators. Phenolic compounds are known as antioxidant for both pollinators and plants. We were interested in comparing plant species in terms of their phenolic compound content: rutin, quercetin, (iso)quercitrin, chlorogenic acid, and hyperoside. Their nectar was obtained from both plant species in 2024 in Ljubljana and the area surrounding Ljubljana. We took 96 samples of each species. The nectar was sampled with microcapillary tubes and analysed by HPLC. When studying the influence of abiotic factors on the concentration of phenolic compounds, the correlations were weak, so we cannot say with certainty which environmental factors affect which phenolic compounds and in what way. Rutin is predominant in the nectar of and chlorogenic acid is predominant in the nectar of . Hyperoside is found in the lowest concentration in both plant species. We found that although secreted much less nectar at midday, it was more concentrated in phenolic compounds at this time than in the morning. In , nectar secretion was highest in the morning, and the concentration of phenolic compounds was also highest in the morning.
雅克氏(Jacq.)和L. 是斯洛文尼亚常见的春季开花物种。它们生长在大片区域,有时会在未管理的草甸中蔓延生长。它们是蜜蜂和其他传粉者的优质花蜜来源。酚类化合物对传粉者和植物来说都是已知的抗氧化剂。我们感兴趣的是比较这两种植物物种在酚类化合物含量方面的差异:芦丁、槲皮素、(异)槲皮苷、绿原酸和金丝桃苷。2024年,我们在卢布尔雅那及其周边地区从这两种植物物种中获取了花蜜。每种物种我们采集了96个样本。用微量毛细管采集花蜜,并通过高效液相色谱法进行分析。在研究非生物因素对酚类化合物浓度的影响时,相关性较弱,所以我们无法确定哪些环境因素会影响哪些酚类化合物以及以何种方式影响。芦丁在雅克氏植物的花蜜中占主导地位,绿原酸在L. 植物的花蜜中占主导地位。金丝桃苷在这两种植物物种中的浓度最低。我们发现,尽管雅克氏植物在中午分泌的花蜜要少得多,但此时其酚类化合物的浓度比早晨更高。在L. 植物中,花蜜分泌在早晨最高,酚类化合物的浓度在早晨也最高。