Genome-Wide Identification and Characterization of the Class III Peroxidase Gene Family in Radish () with Insights into Their Roles in Anthocyanin Metabolism.

Class III peroxidases (PODs) are plant-specific enzymes that play crucial roles in plant growth, development and responses to stress. However, the gene family in the radish ( L.) has not been comprehensively investigated to date. In this study, a total of 95 were identified in the radish genome, which were classified into six subgroups based on a phylogenetic analysis. The gene structures and conserved motifs of the were highly conserved within each subgroup. An intraspecific collinearity analysis revealed 7 tandem and 40 segmental duplication events. An expression analysis across diverse tissues and developmental stages demonstrated that the were functionally involved in radish development. Using a chimeric-colored radish mutant, this study revealed significantly higher POD activity in the green tissues compared to purple tissues. Through transcriptome sequencing, two genes ( and ) were identified as candidate genes related to the anthocyanin metabolism. Our study provides a genome-wide perspective on the genes in the radish and highlights their potential roles in the anthocyanin metabolism. These findings establish a critical foundation for future research aimed at uncovering the functional roles of specific genes, with a particular emphasis on elucidating the molecular mechanisms that regulate anthocyanin degradation in the radish.