Wojdasiewicz Piotr, Wróbel Edyta, Stolarczyk Krzysztof, Stolarczyk Elżbieta U, Mikulska Agnieszka, Szukiewicz Dariusz
Department of Biophysics, Physiology and Pathophysiology, Faculty of Health Sciences, Medical University of Warsaw, 5 Chałubińskiego Street, 02-004 Warsaw, Poland.
Faculty of Chemistry, University of Warsaw, 1 Pasteura Street, 02-093 Warsaw, Poland.
Int J Mol Sci. 2025 Jun 24;26(13):6075. doi: 10.3390/ijms26136075.
Rosavin, a glycoside isolated from , exhibits various biological activities, including potential modulation of metabolic pathways. Despite promising findings in animal models, its effects on many human bone cells remain unexplored. This study aimed to investigate, for the first time, the in vitro effects of rosavin on human osteoblasts (HOBs), focusing on BMP-2 expression, cell morphology, and culture confluence as indicators of osteogenic activity. HOB cultures were treated with 50 µM or 100 µM rosavin for 21 days. BMP-2 expression was measured by ELISA, collagen production was assessed via Sirius Red staining, and cell morphology and confluence were evaluated using phase-contrast microscopy. A significant increase in BMP-2 expression was observed in the 100 µM rosavin group compared to the mineralization control ( < 0.05), particularly on days 14 and 21. Both rosavin-treated groups exhibited higher confluence than controls, with the 50 µM group showing unexpectedly greater confluence than the 100 µM group. Rosavin at 50 µM also promoted a cuboidal morphology characteristic of active HOBs. The presence of collagen validated both the successful progression of the mineralization process and the correct implementation of the experimental protocol. Rosavin enhances BMP-2 expression and supports HOB proliferation and morphological maturation in vitro. These findings suggest its potential as a supportive agent in the prevention or treatment of metabolic bone diseases. Further research is necessary to determine its bioavailability, safety profile, and therapeutic relevance in clinical settings.
迷迭香酸苷是从[具体植物名称未给出]中分离出的一种糖苷，具有多种生物活性，包括对代谢途径的潜在调节作用。尽管在动物模型中取得了令人鼓舞的研究结果，但其对许多人类骨细胞的影响仍未得到探索。本研究首次旨在探讨迷迭香酸苷对人成骨细胞（HOB）的体外作用，重点关注骨形态发生蛋白-2（BMP-2）表达、细胞形态和培养融合度，将其作为成骨活性的指标。用50 μM或100 μM迷迭香酸苷处理HOB培养物21天。通过酶联免疫吸附测定法（ELISA）测量BMP-2表达，通过天狼星红染色评估胶原蛋白生成，并使用相差显微镜评估细胞形态和融合度。与矿化对照组相比，在100 μM迷迭香酸苷组中观察到BMP-2表达显著增加（P<0.05），特别是在第14天和第21天。两个迷迭香酸苷处理组的融合度均高于对照组，50 μM组的融合度意外地高于100 μM组。50 μM的迷迭香酸苷还促进了活跃HOB特有的立方形形态。胶原蛋白的存在证实了矿化过程的成功进展以及实验方案的正确实施。迷迭香酸苷在体外可增强BMP-2表达，并支持HOB增殖和形态成熟。这些发现表明其在预防或治疗代谢性骨病方面具有作为辅助剂的潜力。有必要进行进一步研究以确定其生物利用度、安全性概况以及在临床环境中的治疗相关性。
