Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, 15 Karnjanavanich Road, Songkhla 90110, Thailand.
Int J Food Microbiol. 2013 Oct 15;167(2):229-35. doi: 10.1016/j.ijfoodmicro.2013.09.009. Epub 2013 Sep 21.
A novel poly(lactic acid) (PLA)/sawdust particle (SP) biocomposite film with anti-listeria activity was developed by incorporation of pediocin PA-1/AcH (Ped) using diffusion coating method. Sawdust particle played an important role in embedding pediocin into the hydrophobic PLA film. The anti-listeria activity of the PLA/SP biocomposite film incorporated with Ped (PLA/SP+Ped) was detected, while no activity against the tested pathogen was observed for the control PLA films (without SP and/or Ped). Dry-heat treatment of film before coating with Ped resulted in the highest Ped adsorption (11.63 ± 3.07 μg protein/cm(2)) and the highest anti-listeria activity. A model study of PLA/SP+Ped as a food-contact antimicrobial packaging on raw sliced pork suggests a potential inhibition of Listeria monocytogenes (99% of total listerial population) on raw sliced pork during the chilled storage. This study supports the feasibility of using PLA/SP+Ped film to reduce the initial load of L. monocytogenes on the surface of raw pork.
采用扩散涂层法，将肠球菌素 PA-1/AcH（Ped）掺入新型聚乳酸（PLA）/木屑颗粒（SP）生物复合膜中，开发出具有抗李斯特菌活性的生物复合膜。木屑颗粒在将肠球菌素嵌入疏水性 PLA 膜中起着重要作用。检测到掺入 Ped 的 PLA/SP 生物复合膜（PLA/SP+Ped）具有抗李斯特菌活性，而对照 PLA 膜（无 SP 和/或 Ped）则对测试病原体没有活性。在用 Ped 涂层之前对膜进行干热处理会导致最高的 Ped 吸附（11.63 ± 3.07 μg 蛋白/cm²）和最高的抗李斯特菌活性。PLA/SP+Ped 作为一种与食品接触的抗菌包装在生切片猪肉上的模型研究表明，在冷藏储存过程中，PLA/SP+Ped 对生切片猪肉上的单核细胞增生李斯特菌（总李斯特菌种群的 99%）具有潜在的抑制作用。这项研究支持使用 PLA/SP+Ped 膜来减少生猪肉表面单核细胞增生李斯特菌初始负荷的可行性。
