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小梁钛结构促进人间充质干细胞增殖和骨分化。

Trabecular Titanium Architecture Drives Human Mesenchymal Stem Cell Proliferation and Bone Differentiation.

作者信息

Caliogna Laura, Berni Micaela, Gastaldi Giulia, Grassi Federico Alberto, Jannelli Eugenio, Mosconi Mario, Salatin Elisa, Burelli Silvia, Toninato Riccardo, Pressacco Michele, Pasta Gianluigi

机构信息

Orthopedics and Traumatology Clinic, IRCCS Policlinico San Matteo Foundation, 27100 Pavia, Italy.

Department of Molecular Medicine, University of Pavia, 27100 Pavia, Italy.

出版信息

Int J Mol Sci. 2025 Jul 1;26(13):6354. doi: 10.3390/ijms26136354.

DOI:10.3390/ijms26136354
PMID:40650131
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12250353/
Abstract

The aim of this study is to investigate the adhesion, proliferation, and differentiation of human adipose-derived mesenchymal stem cells (hASC) on Trabecular Titanium scaffolds manufactured with different manufacturing processes (EBM and SLM). The adhesion and proliferation of hASC on titanium scaffolds with WST assays have been carried out. The comparison of the gene expression profiles of typical bone genes (, , , and ) through real-time PCR assays and the evaluation of extracellular matrix composition with immunofluorescence and SEM analysis have been performed. In addition, the possible osteoinductive properties of the two scaffolds have been investigated through real-time PCR and ALP assays. Data showed that Trabecular Titanium supports human adipose-derived mesenchymal stem cell colonization and induces differentiation in bone with the deposition of the abundant extracellular mineralized matrix regardless of the manufacturing process, proving that the micro- and macro-design features are the key factors responsible for the osteoinduction behavior. These features can only be achieved through tailored 3D printing process parameters.

摘要

本研究的目的是调查人脂肪来源间充质干细胞（hASC）在采用不同制造工艺（电子束熔炼和选择性激光熔化）制造的小梁钛支架上的黏附、增殖和分化情况。已通过WST检测法对hASC在钛支架上的黏附和增殖进行了研究。通过实时PCR检测法对典型骨基因（、、、和）的基因表达谱进行了比较，并通过免疫荧光和扫描电子显微镜分析对细胞外基质组成进行了评估。此外，还通过实时PCR和碱性磷酸酶检测法对两种支架可能的骨诱导特性进行了研究。数据表明，无论制造工艺如何，小梁钛都能支持人脂肪来源间充质干细胞的定植，并通过丰富的细胞外矿化基质沉积诱导其向骨分化，证明微观和宏观设计特征是导致骨诱导行为的关键因素。这些特征只能通过定制的3D打印工艺参数来实现。

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