Casagrande Serena, Tiribuzi Roberto, Cassetti Emanuele, Selmin Francesca, Gervasi Gian Luca, Barberini Lanfranco, Freddolini Marco, Ricci Maurizio, Schoubben Aurélie, Cerulli Giuliano G, Blasi Paolo
a Dipartimento di Scienze Farmaceutiche , Università degli Studi di Perugia , Perugia , Italy.
b Laboratorio di Biologia e Medicina Rigenerativa , Istituto di Ricerca Traslazionale per l'Apparato Locomotore Nicola Cerulli-LPMRI , Arezzo , Italy.
Artif Cells Nanomed Biotechnol. 2018;46(sup1):219-229. doi: 10.1080/21691401.2017.1417866. Epub 2017 Dec 21.
In recent decades, tissue engineering strategies have been proposed for the treatment of musculoskeletal diseases and bone fractures to overcome the limitations of the traditional surgical approaches based on allografts and autografts. In this work we report the development of a composite porous poly(dl-lactide-co-glycolide) scaffold suitable for bone regeneration. Scaffolds were produced by thermal sintering of porous microparticles. Next, in order to improve cell adhesion to the scaffold and subsequent proliferation, the scaffolds were coated with the osteoconductive biopolymers chitosan and sodium alginate, in a process that exploited electrostatic interactions between the positively charged biopolymers and the negatively charged PLGA scaffold. The resulting scaffolds were characterized in terms of porosity, degradation rate, mechanical properties, biocompatibility and suitability for bone regeneration. They were found to have an overall porosity of ∼85% and a degradation half time of ∼2 weeks, considered suitable to support de novo bone matrix deposition from mesenchymal stem cells. Histology confirmed the ability of the scaffold to sustain adipose-derived mesenchymal stem cell adhesion, infiltration, proliferation and osteo-differentiation. Histological staining of calcium and microanalysis confirmed the presence of calcium phosphate in the scaffold sections.
近几十年来，人们提出了组织工程策略来治疗肌肉骨骼疾病和骨折，以克服基于同种异体移植和自体移植的传统手术方法的局限性。在这项工作中，我们报告了一种适用于骨再生的复合多孔聚（dl-丙交酯-共-乙交酯）支架的开发。支架是通过多孔微粒的热烧结制备的。接下来，为了改善细胞对支架的粘附及随后的增殖，在利用带正电荷的生物聚合物与带负电荷的聚乳酸-乙醇酸共聚物（PLGA）支架之间的静电相互作用的过程中，用具有骨传导性的生物聚合物壳聚糖和海藻酸钠对支架进行了涂层处理。对所得支架的孔隙率、降解速率、力学性能、生物相容性和骨再生适用性进行了表征。发现它们的总孔隙率约为85%，降解半衰期约为2周，被认为适合支持间充质干细胞从头开始沉积骨基质。组织学证实了支架维持脂肪来源的间充质干细胞粘附、浸润、增殖和骨分化的能力。钙的组织学染色和微量分析证实了支架切片中存在磷酸钙。
