Ji Wantang, Wang Wei, Chen Xingguang, Sun Mingxue, Zhang Jiahua, Nan Bo, Li Xia, Wang Yu, Wang Yuhua, Piao Chunhong
College of Food Science and Engineering, Jilin Agricultural University, Changchun 130033, China; Jilin Province Innovation Center for Food Biological Manufacture, Jilin Agricultural University, Changchun 130033, China.
College of Food Science and Engineering, Jilin Agricultural University, Changchun 130033, China; Jilin Province Innovation Center for Food Biological Manufacture, Jilin Agricultural University, Changchun 130033, China.
Bioresour Technol. 2025 Nov;435:132957. doi: 10.1016/j.biortech.2025.132957. Epub 2025 Jul 10.
Phaffia rhodozyma presents a sustainable alternative to synthetic astaxanthin production, however, its industrial scalability is limited by low yields and unresolved mechanistic ambiguities. This review systematically examines the characteristics of P. rhodozyma, its astaxanthin biosynthetic pathway, and the current advancements of strain development. It critically evaluates existing strategies for enhancing astaxanthin production, with particular emphasis on the application of stress factors during fermentation processes. Furthermore, the mechanisms underlying stress-induced astaxanthin accumulation are comprehensively elucidated, encompassing oxidative stress-driven astaxanthin synthesis, enhancing precursor supply through regulation of central carbon metabolic pathways, as well as competitive pathways, and esterification-mediated alleviation of feedback inhibition. The stress-induced synergistic regulation of multiple mechanisms is systematically analyzed, along with a rational framework for the selection and application of stress factors. This in-depth analysis provides actionable insights to facilitate the transition from laboratory-scale studies to industrial applications of P. rhodozyma.
红发夫酵母是合成虾青素生产的一种可持续替代方案，然而，其工业可扩展性受到低产量和未解决的机制模糊性的限制。本综述系统地研究了红发夫酵母的特性、其虾青素生物合成途径以及菌株开发的当前进展。它批判性地评估了提高虾青素产量的现有策略，特别强调了发酵过程中应激因素的应用。此外，全面阐明了应激诱导虾青素积累的机制，包括氧化应激驱动的虾青素合成、通过调节中心碳代谢途径以及竞争途径增强前体供应，以及酯化介导的反馈抑制缓解。系统分析了应激诱导的多种机制的协同调节，以及应激因素选择和应用的合理框架。这一深入分析提供了可操作的见解，以促进从实验室规模研究向红发夫酵母工业应用的转变。
