Sun Meixue, Jiang Wenhui, Liu Yingjie, Wang Jun, Sun Zheng, Ren Guangwei, Xu Pengjun, Deng Haibin
Tobacco Research Institute of Guangdong Province, Shaoguan, China.
Key Laboratory of Tobacco Pest Monitoring Controlling & Integrated Management, Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao, China.
Arch Insect Biochem Physiol. 2025 Jul;119(3):e70074. doi: 10.1002/arch.70074.
Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are essential for host-seeking behaviors in herbivorous and predatory insects. However, limited studies have examined differences in the OBP and CSP profiles between herbivores and predators. Mirid bugs, which ancestrally were predatory, provide an ideal model to investigate these differences because some of mirid species have evolved to develop herbivorous capacities. In this study, we compared compared OBP and CSP profiles among transcriptome analysis of one zoophytophagous and four phytozoophagous mirid bugs, identifying a total of 146 OBPs and 69 CSPs. The number of OBPs was similar between phytozoophagous (26 in Adelphocoris fasciaticollis, 29 in Adelphocoris lineolatus, 28 in Apolygus lucorum, and 32 in Adelphocoris suturalis) and zoophytophagous (31 in Nesidiocoris tenuis) species. However, the zoophytophagous specie had more CSPs than the phytozoophagous species. Phylogenetic analysis revealed two types of OBPs in mirid bugs, with classic OBPs being more abundant than plus-C OBPs. Transcriptome revealed that the number of OBPs with high expressions was higher in the phytozoophagous species (18) than in the zoophytophagous species (14). By contrast, more CSPs (10) exhibited high expressions in the zoophytophagous mirid bug compared with those in the phytozoophagous species (≤ 7). These results suggest that differences in the number and expression levels of OBPs and CSPs between zoophytophagous and phytozoophagous mirid bugs affect host-selection processes. In conclusion, the findings provide a basis for developing novel pest management strategies, including the use of natural enemies or behavioral regulation of mirid bugs through OBP and CSP gene manipulation.
气味结合蛋白（OBPs）和化学感受蛋白（CSPs）对于植食性和捕食性昆虫寻找宿主的行为至关重要。然而，对植食性昆虫和捕食性昆虫之间OBP和CSP谱差异的研究有限。盲蝽科昆虫最初是捕食性的，是研究这些差异的理想模型，因为一些盲蝽物种已经进化出了植食能力。在本研究中，我们比较了一种杂食性盲蝽和四种植食性盲蝽转录组分析中的OBP和CSP谱，共鉴定出146种OBP和69种CSP。植食性盲蝽（苜蓿盲蝽有26种、中黑盲蝽有29种、绿盲蝽有28种、苜蓿盲蝽有32种）和杂食性盲蝽（烟盲蝽有31种）的OBP数量相似。然而，杂食性盲蝽的CSP比植食性盲蝽多。系统发育分析揭示了盲蝽科昆虫中有两种类型的OBP，经典OBP比加C OBP更丰富。转录组显示，高表达OBP的数量在植食性盲蝽（18种）中高于杂食性盲蝽（14种）。相比之下，与植食性盲蝽（≤7种）相比，杂食性盲蝽中有更多的CSP（10种）表现出高表达。这些结果表明，杂食性盲蝽和植食性盲蝽之间OBP和CSP数量及表达水平的差异影响宿主选择过程。总之，这些发现为制定新的害虫管理策略提供了基础，包括利用天敌或通过OBP和CSP基因操纵对盲蝽进行行为调控。
