Aydin Elif Burcu
Scientific and Technological Research Center, Tekirdağ Namık Kemal University, Tekirdağ, Turkiye.
Turk J Chem. 2025 Apr 29;49(3):382-393. doi: 10.55730/1300-0527.3737. eCollection 2025.
An ultrasensitive impedimetric immunosensor was fabricated using a poly(glycidyl methacrylate) (PGMA) polymer-covered indium tin oxide (ITO) platform for the quantification of kallikrein 4 (KLK4), an important prostate cancer biomarker. PGMA had suitable biocompatibility and nontoxicity for loading of antiKLK4 antibodies on the ITO substrate surface. Anti-KLK4 biomolecules were directly attached to the PGMA-covered electrode surface via epoxy groups of the PGMA polymer. The preparation method for the PGMA matrix-modified electrode was simple and inexpensive. The proposed biosensor immobilization layers coated on the ITO electrode were characterized with electrochemical techniques. The experimental parameters that affect biosensor response were optimized, and the suggested sensor showed a linear response from 0.04 pg/mL to 8 pg/mL with a low detection limit (LOD) of 12.21 fg/mL. Moreover, it had acceptable stability, reproducibility, and repeatability. Additionally, the disposable biosensor offered excellent reliability and accuracy in KLK4 analysis, suggesting that it could be used as an alternative technique in clinical diagnosis.
利用聚甲基丙烯酸缩水甘油酯（PGMA）聚合物包覆的氧化铟锡（ITO）平台制备了一种超灵敏阻抗免疫传感器，用于定量检测激肽释放酶4（KLK4），这是一种重要的前列腺癌生物标志物。PGMA具有合适的生物相容性和无毒性，可用于在ITO基底表面负载抗KLK4抗体。抗KLK4生物分子通过PGMA聚合物的环氧基团直接附着在PGMA包覆的电极表面。PGMA基质修饰电极的制备方法简单且成本低廉。采用电化学技术对涂覆在ITO电极上的所提出的生物传感器固定层进行了表征。优化了影响生物传感器响应的实验参数，所建议的传感器在0.04 pg/mL至8 pg/mL范围内呈现线性响应，检测限低至12.21 fg/mL。此外，它具有可接受的稳定性、重现性和重复性。此外，这种一次性生物传感器在KLK4分析中具有出色的可靠性和准确性，表明它可作为临床诊断中的一种替代技术。
