Xu Weifan, Xue Zhaolong, Xie Ge, Xi Hongbo, Yang Hui, Lou Yi, Zhao Mingzhi, Wang Qinye
Department of Orthopaedics, Nanxiang Branch of Ruijin Hospital, Shanghai 201802, China.
Department of Orthopedics, Xie's Gukang Hospital of Yongshun County, Xiangxi autonomous prefecture, Hunan 416700, China.
ACS Omega. 2025 Jun 26;10(26):28065-28074. doi: 10.1021/acsomega.5c02130. eCollection 2025 Jul 8.
Due to the differences in cell types, structural composition, and mechanical properties between articular cartilage and subchondral bone, developing effective integrated repair strategy persists as a major clinical hurdle. We constructed a novel scaffold by a three-dimensional (3D) printing and surface modification method for the potential application of simultaneously promoting bone and cartilage repair. MgAl-layered double hydroxide (MgAl-LDH) nanosheets were synthesized via the hydrothermal method and then modified on β-tricalcium phosphate (β-TCP) scaffolds. The prepared composite scaffolds exhibited a uniform microstructure, suitable compressive strength, and satisfactory biocompatibility. Attributing to the released bioactive ions, MgAl-LDHs-TCP effectively supported the proliferation and attachment of rabbit bone marrow mesenchymal stem cells and chondrocytes. Furthermore, 72MgAl-LDHs-TCP scaffolds obviously promoted osteogenic differentiation, provided deposition sites for calcium salt formation, induced the formation of alkaline phosphatase, and created favorable conditions for the early-stage and end-stage osteogenic differentiation of rBMSCs. Simultaneously, attributed to the released functional ions, the prepared composite scaffolds significantly enhanced the synthetic metabolism in chondrocytes and inhibited the expression of catabolism-related genes, playing an important role in protecting arthritis chondrocytes. Overall, such prepared scaffolds may have the possible application for the simultaneous regeneration of cartilage and subchondral bone defects.
由于关节软骨和软骨下骨在细胞类型、结构组成和力学性能方面存在差异，制定有效的综合修复策略仍然是一个主要的临床障碍。我们通过三维（3D）打印和表面改性方法构建了一种新型支架，用于同时促进骨和软骨修复的潜在应用。通过水热法合成了MgAl层状双氢氧化物（MgAl-LDH）纳米片，然后在β-磷酸三钙（β-TCP）支架上进行改性。制备的复合支架具有均匀的微观结构、合适的抗压强度和令人满意的生物相容性。由于释放的生物活性离子，MgAl-LDHs-TCP有效地支持了兔骨髓间充质干细胞和软骨细胞的增殖和附着。此外，72MgAl-LDHs-TCP支架明显促进了成骨分化，为钙盐形成提供了沉积位点，诱导了碱性磷酸酶的形成，并为rBMSCs的早期和成骨分化创造了有利条件。同时，由于释放的功能离子，制备的复合支架显著增强了软骨细胞的合成代谢并抑制了分解代谢相关基因的表达，在保护关节炎软骨细胞方面发挥了重要作用。总体而言，这种制备的支架可能在软骨和软骨下骨缺损的同时再生方面具有应用潜力。
